In MTT test we can measure the toxicity of plant crude extracts, at which concentration we can consider the crude extract with potent, remarkable, good, weak or no activity? is there a scale according NCI "national cancer institute"
Dear Ammar,
You have to measure protein levels in extracts of medicinal plants. based on the protein content of the tests carried out, whether or not toxic at concentrations less than 500 ug / mL
good luck,
AL
Hello Ammar,
i agreed with previous comments.
If you are using a crude extract then you can go up-to 1 mg/ml concentration to check for cell viability, but remember one thing simultaneously you have to check toxic effect of sample on normal cell line (non cancerous cells).
suppose that, if your sample is up to 100 ug / mL is not toxic to normal cells (that means cell viability more than 90% compare to untreated cells), in that case you can use less than 100ug / mL of your sample on cancer cell lines to check its anti-cancerous effect, and later stage you can go for IC50 concentration.
Hope so it may helpful to your research.
Good day.
The most ideal method to determine cytotoxicity potential is by measured the IC50 value. However, the IC50 values that are obtained by MTT assay can also be validated by 1 or 2 other endpoints such as trypan blue (vital staining). Also, the IC50 value of a particular compound will vary among cell lines.
One can start off with a certain range of concentration initially and based on the % cell killing results obtained, the concentrations for obtaining IC50 values can be standardized.
It is ideal to use at least 2 endpoints in 2 different cell lines to report cytotoxic potential of a novel compound being reported for the first time.
hye Mudasir. If there any original reference on what have been you mention above? i search on the website. but i did not found it. thanks
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