I am staining for Pax7 (fluorescence), and was looking for a negative control to establish the staining protocol. Any suggestion would be highly appreciated.
Why not just to do CRISPR k.o. in your existing cells? You can do the assembly of lentiviral vector in HEK293T and then just infect your C2C12. I have not worked with C2C12 cells but if they are easily transfectable you can just transfect them with CRISPR construct itself without lentiviral assembly.