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Questions related to Cancer Research
I'm working with HEK293 cells stably expressing a lentiviral CMV-FL construct that responds to luciferase when plated.Does anyone know if these same cells would still luminescence while in a...
30 September 2016 801 2 View
It should cross-link upon UV-light exposure but cleavable by DTT or something else. I need it to cross-link proteins and perform mass spectrometry to identify binding partners. I used another...
30 September 2016 4,777 8 View
I model the population dynamics of several (adherent) cell lines. To that aim I want to characterize the growth dynamics of those lines experimentally. We already measured the growthrate (=birth -...
30 September 2016 5,054 3 View
I am trying to reduce noise in a single-channel recording experiment. I currently have 1 pA noise. I am thinking to use a heavier gauge wire to connect the ground bus (a copper bar) in the air...
29 September 2016 1,898 4 View
hey everyone and thanks in advance for your help! We are clueless about this: I am looking at treatment response to fulvestrant and tamoxifen in breast cancer cells with specific mutations. for...
28 September 2016 8,505 2 View
I am looking for a retrograde tracer to inject in the bone morrow for labelling of sensory afferents in the DRG. It should be stable for at least 3 weeks as it will be combined with another...
28 September 2016 8,366 1 View
The DNA acquires mutation over the replication cycles, and once one mutation is propagated then that never gets fixed. Can we develop a method to reverse that process? as de differentiation of...
28 September 2016 6,574 3 View
I am trying to understand how to calculate the Big O i.e. the spatial complexity of Image steganography Techniques but I did not find enough information to understand this topic, can someone...
27 September 2016 6,383 1 View
I have noticed these cells which seem considerably larger than the majority of the cells in my tissue culture flasks for NCTC clone 929 and LN-229 cells. The media for the flasks looks normal and...
27 September 2016 1,717 3 View
I am performing qPCR to determine differential expression of certain miRNAs in the serum of liver cancer patients, and was wondering if anyone used miR-16 as an endogenous control before?
26 September 2016 9,424 2 View
I am using propidium iodide (Hi-media Product) but i want to know how much Concentration of propidium iodide is required to stain in 100 cells ?
26 September 2016 1,327 2 View
Research question: Does Non-pharmaceutical intervention (Tai Chi, Qigong, light exercise... ) effect on symptom clusters such as cluster 1 (Fatigue, dyspnea, and cough) or cluster 2 (Fatigue,...
19 September 2016 9,323 3 View
I am using 1M NaOH solution to dissolve a drug. I am trying to estimate its IC50 using MTT assay. The data sheet says that the drug is soluble in 1M Naoh at 50mg/ml. Has anyone had experience...
19 September 2016 1,129 6 View
Does the Phoenix-Ampho packaging cell line expresses the 10A1 viral envelope?
18 September 2016 8,240 2 View
The incubation period of the disease in which the patient has no external symptoms and the disease does not affect a person's life.
18 September 2016 7,996 3 View
I'm looking for a marker specific to T helper 3 cells (Th3) for use in a multicolor flow cytometry panel. Any suggestions for a specific marker, or combination of markers, is greatly appreciated.
18 September 2016 3,243 2 View
I want to do a cell cycle synchronization of mouse ESCs in M phase. I need to do a mitotic shake-off and re-plate my cells, and follow progression of the cell cycle. The protocol I found suggests...
16 September 2016 7,150 1 View
In treatment of non-small cell carcinoma differentiating adeno from squamous cell carcinoma is essential.When morphological diagnosis is difficult IHC is done and sometimes we get adeno and...
15 September 2016 6,468 4 View
Hi, During our experiments we noticed that nude mice orthotopically grafted with GBM, develop cutaneous pustules when treated with TMZ (schedule: 1 week on and 2 weeks off for 6 cycles). We...
15 September 2016 6,743 4 View
I am working with exosomes purified from whole saliva samples. I would like to know the best RNA kit to isolate both mRNA and miRNA from exosomes for microarray profiling. I appreciate any response.
14 September 2016 1,326 5 View
I am conducting a study on knowledge, attitude and perception of women on breast cancer. I need a validated questionnaire targeting all these 3 areas. Can anyone help?
14 September 2016 2,500 1 View
Electronically frequency and amplitude can be maintained for longer duration and both parameters may be adjusted independently
13 September 2016 8,781 1 View
Hello, I need a help with IC50 determination in Excel. Usually I am doing it in sigmaplot or GraphPad usin four-parametr nonlinear regression, but now I must do it it Excel. I have seen some...
13 September 2016 367 8 View
We know that in the CRISPR Cas9 system, sgRNA and cas9 together cleave/silence/block/bind areas of interest in the genome. However, would sgRNA alone (without the cas9) be able to Target the gene...
13 September 2016 4,339 3 View