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Questions related to Cancer Research
Dear All, I'd like to characterize 2 mouse cell lines in terms of mutations and would like to know if you had good primers and protocol (or any other way to test it) to test the following...
24 April 2017 9,946 3 View
Is there any difference when I find Monocytic or Granulocytic myeloid-derived suppressor cells ( MDSCs ) in the tumours? Thanks
18 April 2017 2,129 1 View
We are studying with celastrol about prostate cancer
15 April 2017 370 2 View
would you recommend adjuvant chemotherapy for t1 t2 no colon cancer with poor prognostic factors eg poorly differentiated or lymphovascukar invasion any evidence for an against
15 April 2017 1,591 5 View
Any suggestion on an essay I can use to separate pancreatic and prostate primary cells growing together ? or an antibody that specific to pancreatic cell and not prostate cells and vice versa ?
11 April 2017 8,238 4 View
Hello I'm looking for a lung cancer cell line. I'll use NCI-H1299 cells for my experiments, but it has partial deletion in p53 and lacks expression of p53 protein. Because I wanted to consider the...
11 April 2017 2,327 3 View
Besides MTT based assays what else are the available assays to evaluate drug dose for killing of cancer cell lines.
07 April 2017 1,729 5 View
If so what did you measure (e.g. NF-kB)
06 April 2017 7,219 2 View
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04 April 2017 8,855 2 View
I've created stable cell lines that have a depletion of a protein that I study. I would like to survey these cells to look for increases or decreases relative to the wild type or scrambled...
31 March 2017 8,295 1 View
I am intending to stabilise HIF 1 aplha expression in cancer cells; I mean to say to induce hypoxia mimetic environment. Since we don't have a hypoxic chamber availability, we are intending to go...
31 March 2017 1,537 5 View
I would like to know if there's a separate database for variants that have been reported from stem cells?It could even be a tissue-specific variant database where the tissue type or subtype is...
28 March 2017 6,700 2 View
In many findings or research articles researchers says that their molecules changed the expression of BCl2, Bax, PARP, p53,p21 etc. but in true sense how a molecules (even tiny) can cahnge the...
21 March 2017 3,163 3 View
Tumor suppressor genes (such as Rb, APC, PTEN and the ever famous p53) are often downregulated in cancer cells, if not completely lost or dysfunctional. I'd like to know if there are any tumor...
07 March 2017 8,033 3 View
Dear collegues could somebody explain to me what is the meaning of TDC (Tummor dissociation constant) in colorectal and pancreatic cancers. Thanks a lot!
06 March 2017 8,215 1 View
I am expecting most reliable and frequently mutated genes for Breast Cancer in Indian Population. Is text mining the only option?
02 March 2017 5,150 1 View
I am setting up an experimental metastatic model of Lewis lung carcinoma by intravenous injection of LL/2 cells into the tail vein. I am looking for ways to quantify tumor burden in the lungs. I...
28 February 2017 8,052 2 View
what combination of chemotherapeutic agents for trimodal therapy of bladder cancer (MIBC) are more effectives? Is resonable to use MVAC?
25 February 2017 1,771 1 View
We have been trying to develop 4T1 breast tumor model in balb/c mice. We have been injected 10^6 4T1 cells suspended in 100 uL PBS into the mammary fad pad region, subcutaneously. After 10 days we...
25 February 2017 6,630 2 View
Gene expression in the liver is zonated , is during aging a shift in zonation possible of the expressed genes?
25 February 2017 3,153 3 View
I've done RT-PCR for TP53 gene and the results showed an increase of the gene expression comparing to its normal match and as known TP53 is a tumor suppressor gene which should be down regulated...
24 February 2017 4,994 9 View
I was planning to dock some isolated compounds with the Estrogen receptor associated with breast cancer and found 3 known receptors viz. 3ERT, 2YLY, 2IOK.which receptor should I use and why?
20 February 2017 790 3 View
TMZ is FDA approved for GBM in humans but its overall effectiveness is limited. In intracranial mouse xenografts of run-of-the mill GBM cell lines like U87, how well does TMZ perform? I.e. is it...
17 February 2017 1,044 5 View
I use 3-methyl adenine at a final concentration of 7 mMol, but I am not convinced about using it because it solidifies and forms aggregates in the media.
15 February 2017 6,948 15 View