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Questions related to Cancer Research
SiHa, HCT116, HT-29, and FaDu cell lines have been widely used in tumour hypoxia studies. Any particular reason(s) for selecting those lines? Thanks everyone :)
02 June 2016 6,306 2 View
Many thanks.
01 April 2016 9,422 3 View
In my job, I transient transfected Active-wnt3a construct into Hek293T and Hek293 for expression , I collected three batch(fresh medium 5ml in 10cm dish for 24hr) conditioned medium...
24 February 2016 9,471 5 View
Actually, we are using a CT26 colon cancer model but it is very aggressive and our vaccine is not working well when compared to EG7 model. We need to set up a colon cancer model that is less...
18 February 2016 3,157 1 View
Read 8 answers by scientists to the question asked by Jason Davenport on Feb 14, 2016
14 February 2016 3,674 8 View
I wanted to start a project to test drugs on CLL cells. at this point i dont want to use sample from hospitals; therefore, I would like to know which commercial leukemia cell lines should be the...
30 January 2016 9,689 3 View
BEIR VII, cancer risk, LAR
09 January 2016 5,154 4 View
01 January 2016 5,884 4 View
While validating the mode of action of a novel anti-cancer compound, we have options of using small interference RNA or pharmacological inhibitors of a particular signaling molecule. In several...
01 January 2016 1,252 5 View
When reading articles using the Charlson Comorbidity Score (Charlson ME et al J Chron Dis. 1987;40:373–383) to describe the comorbidity of a cohort of patients with cancer, for instance head neck...
12 December 2015 7,937 4 View
I'm doing cancer research and I want to model the risk of having a cancer recurrence on various sites in the body, with other sites and death as competing risks. My variables are: diagdate ("Date...
12 December 2015 5,632 1 View
in cancer research, target gene's function is tested by overexpression or knockdown assay, but the result is different in use of transient and stable transfection, how to choose? and which is...
12 December 2015 3,380 8 View
Hi all, To conduct a cancer based discovery study by NGS what is the optimal number of samples [minimum number to be precise] which would yield convincing results as it is difficult to sponsor for...
11 November 2015 6,706 3 View
Hello everyone, I would like to find a database available on Internet to select a population of GBM patients who display the EGFR V3 mutation. An in this cohort of patients, I would like also to...
10 October 2015 2,377 7 View
I have injected 1,2 dimehylhydrazine (40 mg/kgBW/weekly once) subcutaneously to male wistar rats to develop colon cancer rat model up to 8 doses (followed previously reported articles). I have...
09 September 2015 3,758 4 View
I would like to reduce the numbers of BM cells through irradiation and reconstitute with new BM cells, or not. The goal would be to study whether peripheral immune cell precursors are able to...
09 September 2015 441 5 View
I have been trying to standardize the protocol using 1% agarose for the mammosphere assay. I'm observing that even at very low seeding density the cells tend to aggregate or clump together. It...
06 June 2015 600 3 View
In most cases it is reported that copy number alteration of genes drive cancer development but it is also quite a fact that CNA increase the chance of survival of the organism under the imposed...
04 April 2015 3,730 4 View
There might be issues with using Trypsin to remove adherent HCT-116 cells and staining with Annexin V to ananlyze apoptosis using flow cytometry.
04 April 2015 8,109 9 View
I have been trying to show the difference in TGF-treated "activated" fibroblast from untreated mouse embryonic fibroblast (MEF) and according to literature, the difference in alpha smooth muscle...
03 March 2015 4,931 14 View
Several publications suggest there is an association between excess growth hormone and malignancy in patients with acromegaly. For instance, this association has been well described for colon...
02 February 2015 1,139 4 View
Dear colleagues, I've browsed a lot of papers and encountered the fact that when the both primary and metastatic tumors are of interest for researchers and when the xenograft model is involved,...
02 February 2015 3,365 7 View
I am testing various anticancer drugs onto several cell lines. I test the cytotoxicity based on 72hr incubation with drugs and assayed via MTS. I keep on getting low absorbance for negative...
02 February 2015 3,525 37 View
I am targeting bcl2 and bax pathway in breast cancer cells, MCF7 and MDAMb231 and my drug may block this pathway. According to my hypothesis, I will use western blot and qRT-PCR to confirm this...
02 February 2015 630 2 View