How to check the pH of E. coli culture initially? and how to change the pH of the E. coli culture?

I am working on E. coli 15597 prepared in Luria broth. Initially, the pH of LB is 7 after the inoculation of the E. coli it changes to 8.26 is it correct? can bacteria be alkaline?

15 October 2020 2,042 2 View

What are the best pipleline available for the RNA-Seq data analysis ?

What is the latest pipeline to run the RNA-Seq genomic data ?

12 October 2020 7,618 2 View

If an article is unknowingly published in a fake journal, is it considered as published or can the article be sent to some other journal to publish?

How do we handle this menace of fake journals?

14 July 2020 4,835 7 View

How do we differentiate conservation agriculture and regenerative agriculture in context of sustainable farming practice?

Sustainable farming practices appears into different names confusing the global audience which to follow and why? What makes conservation agriculture as different approach than regenerative...

30 June 2020 7,726 2 View

How to get DOI for my published article in a journal which has not given any DOI?

I want to know if Research Gate generates DOI for articles which has been published but has not been provided any DOI by the publisher?

23 May 2020 510 7 View

How many times do we have to check and report model fit measures during SEM?

I am trying to run a SEM which has four latent variables in it. Let us consider these variables as A, B, C and D. All are latent. Here A and B are independent variables, C is mediator and E is...

17 May 2020 4,091 3 View

What is the criteria to calculat e the plagarism?

What is the criteria to calculat plagarism for writing manuscript

06 April 2020 6,936 3 View

What happens if we lower the number of cycles in explicit dynamics for ansys ?

Hi I am a student pursing my final year in mechanical engineering. I am doing crash analysis of the car frame in Ansys software in explicit dynamics module. I had my analysis running for 2-3 days...

18 March 2020 3,709 3 View

Why contaminant is transporting from lower part to higher part in various column experiments? What is reason behind this?

can we transport the contaminant from the higher part to the lower part of the column?

25 December 2019 7,124 3 View

Can authentication in IoT be implemented using BlockChain techniques?

Authentication in IoT systems are vastly insecure. Mostly the problems lies on its open ended and probable untrustworthy nodes. Also, the need for a lighter authentication mechanism will always be...

09 December 2019 4,896 4 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Timeout error!! Processor in the loop (PIL) using STM32F4 Matlab 2018a?

Dear Researchers I am trying to perform a PIL simulation using STM32F4 Discovery board and comunication serial USB TO TTL. During simulation I receive the following timeout error: An error...

01 March 2021 2,327 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View