How can i deoxygenate oxyhemoglobin solution using nitrogen gas?

02 January 2024 0 5K Report

I want to deoxygenate oxyhemoglobin with nitrogen bubbling, but i can't observe the characteristic peak of deoxyHb in UV-Vis spectrum.

Also, the concentration of my hemoglobin sample was little high (about 50 mg/ml) so i think the deoxygenation isn't going well because of this.

How can i prepare deoxygenation of solution from oxyhemoglobin with nitrogen purging? Can i ask more detailed protocol of deoxygenation work.

If not, how can i get deoxyHb without usage of chemicals such as Stokes' reagent?

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