In FACS binding analysis, I have seen a increase in percent positivity when I change my final resuspended cell count from 0.1 M to 0.2 M cells?

20 December 2023 0 9K Report

When I was acquiring half of my sample as live cells and half fixed, I used to get 30% positivity and now when Im taking the whole sample as live cells only, it increased to 90%. What is the possible explanation for this?

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Science method

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