I prepared a culture media of L-15 with 20% FCS. After culturing cell for 8 days and feeding I saw these structures. But I'm not understanding this. Wether it can be cell or contamination.
I am a ms student and new in cell culture techniques.
As a former PhD scholar, currently working as a Junior Research Fellow on a DST-funded project, I am seeking information on travel grants. A few months ago, while I was still pursuing my PhD, I...
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Trying to find the relationship between epigenetic and cancer more deeply.
30 December 2023 8,363 2 View
Hello all , I have a two-plate configuration and there is a dielectric in between them . I launch electrons from plate and it reaches another plates through the dielectric . I kept the dielectric...
16 October 2023 5,702 0 View
Hello, I have performed MM-PBSA calculation of a protein-ligand complex. I utilised approx 750 frames (out of a total of 15000 frames) to compute the free enrgy change of binding. Then, I used a...
04 October 2023 247 2 View
primers are short oligonucleotides specifically designed for sequencing and PCR. But if we don't know where to start sequencing for a novel virus's genome then how the primers can be designed? or...
19 September 2023 4,636 3 View
Cancer means the uncontrol cell division, but what factors increase the risk of breast cancer in females other than the genetic issue?
18 September 2023 5,790 4 View
How can I pick up the microplastic particle when doing FTIR-ATR? Beacuse I can not directly place my filter paper under ATR.
01 September 2023 2,004 9 View
I am starting my CHIP qPCR on MCF7 and MDAmb231 cell lines and I am Using the IMPRINT CHIP kit by Sigma. I need to optimize the sonication time for both cell lines, but the buffers are very...
03 August 2023 3,170 0 View
I have a comparative transcriptome dataset, with a number of upregulated and downregulated genes. I want to construct a protein-protein interactome network to understand the node and hub genes and...
23 July 2023 3,286 0 View
Although there are several research papers describing methods to estimate different organic phosphorus fractions, I did not found any comprehensive method to estimate different organic P...
25 June 2023 6,228 0 View
I am using Rhodamine6G as gain medium and silver nanoparticles as scatterers on a microscope slide and laser input 532 nm comes from above.
09 August 2024 9,894 2 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...
05 August 2024 3,783 2 View
Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...
04 August 2024 4,255 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
How Social Media Affects Your Mental Health
04 August 2024 6,961 3 View