I'm planning to detect lipid-related signals on paraffin-embedded tissue sections and have the BODIPY 558/568 C12 lipid probe. I've found that it's commonly used for lipid droplet labeling in live cells or frozen sections, but I haven't found any specific instructions for use on paraffin sections.
Has anyone tried using this dye on deparaffinized and rehydrated tissue sections? Does it penetrate effectively and retain specific signals? Will using it on paraffin sections affect signal stability or produce high background?
BODIPY 558/568 C12 is mainly intended for live-cell or frozen tissue staining, as paraffin processing removes most lipids from the sample. In paraffin sections, the dye usually shows very weak or nonspecific staining because the lipid environment is largely lost.
If you need lipid detection in paraffin-embedded tissue, antibody-based markers for lipid-associated proteins may work better. Alternatively, use frozen sections to preserve lipids for BODIPY staining.
The answer to this question comes from MCE Technical Support.
- Badges
- Science topic