I'm planning to culture lymphocytes isolated from the whole blood. I know that both media are efficient in isolating PBMCs, but I'm wondering which one I should use to isolate PBMCs for culture.
Yes, both media are efficient in isolating PBMCs. You may use Histopaque-1077 when isolating lymphocytes from small volumes of blood. Though yields might be slightly lower compared to Ficoll.
Ficoll is generally considered the more preferred option, particularly when working with larger blood volumes.