What colour is a resorufin solution at pH < 6.1?

The redox dye resazurin (dark blue) can be reduced in aqueous solution at pH > 6.1 via resorufin (pink) to dihydroresorufin (colourless). What colour is the resorufin solution at pH < 6.1?

21 January 2021 8,442 2 View

We use Thermo Scientific Superfrost plus gold coated slides. Why our cryo sections of dry fruits do not stick to the slides?

We use Thermo Scientific Superfrost plus gold coated slides. Why our cryo sections of dry fruits do not stick to the slides?

13 January 2020 1,215 1 View

My mysterious red dye may not be neutralized before HPLC/MS as it is only stable between pH 12.5-12.9. Who could help with equipment tolerating pH13?

More information in https://www.chemistryviews.org/details/education/10821368/150_Years_Alfred_Wohlk.html

04 July 2019 1,409 1 View

Can anyone recommend me any paper about principles of hydrometallurgy?

I would like to know if there are any papers which define and explain principles of hydrometallurgical processes ?

07 January 2019 3,430 3 View

Are smaller insect species more sensitive to residual insectides?

Alarming declines of insects elicited a vivid public debate, particularly in Germany (#Insektensterben), but also in many other countries (see...

06 September 2017 7,975 4 View

Which flower signals are honest?

There are some reports about honest floral signals, e.g. flower colour change in many flowering plants correleted to nectar production, size of Dalechampia bracts indicating the size of a resin...

25 April 2016 1,418 5 View

Which pan trap colors are attractive for bees?

Dear All, I want to learn about pan trapping of bees and the most attractive colours. Does anybody know published spectral reflectance curves of pan trap colors? Can anybody explain the term...

08 March 2016 7,710 44 View

How does flower suntracking correspond to bee foraging behaviour?

Many flowers show heliotropism and the flowers are sun tracking. The discussed benefits of sun tracking flowers are heating up the flowers for better pollen germination and seed production, and...

26 January 2016 9,316 5 View

Does it make sense to compare linear regression slopes in the Murmansk region (Russia) using air temperatures from gridded E-OBS data?

12 June 2015 8,483 14 View

Why do some researchers neglect multiplicity in the calculation of statistical significance?

14 February 2014 786 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Samples to study circular dichroism (chirality)?

I want to build a low-cost polarimeter to study circular dichroism (chirality). What type of samples can I use? I am looking for samples that can be found outside the laboratory.

01 March 2021 9,101 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View