What is the best flow cytometer to analyze microparticles?

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Why is cronbach's alpha sometimes calculated for non-unidimensional questionaries?

I've recently come across with several published articles where questionaries composed of more than one section (where every section is meant to gather data for separate variables/dimensions of...

11 November 2020 1,805 7 View

Could you recommend a Journal to publish a literature review about the relationship between globalization and ultranationalism in Europe?

Currently I am finishing the literature review about the relationship between globalization and the resurgence of ultranationalism parties in Western Europe. This is the first part of my Doctoral...

02 October 2020 9,773 5 View

Where to find data for Frequency Regulation and Peak Shaving control signals for intregration of BESS to the utility grid?

I'm looking to study Battery Energy Storage System (BESS) integration to the utility grid for different applications such as Frequency Regulation and Peak Shaving. I would like to learn about...

05 August 2020 2,075 1 View

Glass luminescence after high electromagnetic pulse?

In a Z-pinch test using Pulsotron-3 fusion reactor, I have seen at high-speed camera that a pyrex glass generated a beautiful green light during some milliseconds after the electromagnetic pulse...

18 July 2020 2,488 2 View

Collaboration against COVID-19 based on 3D printing, any JCR journal suggestion?

Good morning, Our university has collaborated during the last three months of the pandemic with the printing of PPEs by means of 3D printing, contributing with more than 26,000 parts in a...

09 June 2020 7,107 4 View

What is the best intelligent algorithm to control engines?

There are PIDs but usually only the Proportional part of the PID algorithm is usually used Mapping systems, as used in diesel engines But make a several layer PIDs is difficult. Map based systems...

06 May 2020 1,065 0 View

How to calculate total electronic cross sections?

Hello, I'm trying to understand photon shielding parameters such as mass attenuation coefficients (MAC's) and effective atomic numbers (Zeff's). I would like to ask how to calculate for total...

08 April 2020 7,893 2 View

How do you deal with lost data?

Given your specific discipline. Have you ever irretrievably lost data of an ongoing research project? How did you handle it? Thanks in advance. (Also, this is my story. A couple of years ago, in...

29 March 2020 297 21 View

Which environmental problems arise from landfill mining ?

Landfill mining techniques arise as one of the available alternatives to mitigate negative environmental impacts from municipal solid waste landfills. High recycling potential generation is one of...

28 March 2020 1,782 10 View

How should a shapefile (.shp) be referenced?

A local fault shapefile prepared by a public academic institution was required to complement a qualitative analysis. It was not possible to access a study or technical report documenting the data...

11 March 2020 4,329 3 View

What is the characteristic length to determine the Reynolds number for the scattered turbulent fluid flow?

Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...

02 March 2021 1,999 5 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Why do the air flow results vary totally when adding scalar in CFD ?

Dear All, We are using Autodesk cfd to calculate the air flow in a single sided naturally ventilated room, where air flow depends only on buoyancy due to temperature difference between inside and...

25 February 2021 9,602 4 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Can we recognize signs of the "volatility clustering" property of a time series in its time domain plot?

My purpose is to recognize the volatility clustering property without statistical tests such as The Lagrange Multiplier (LM) test.

24 February 2021 4,150 3 View

Why anti-CD20 or other antibodies are not used to label target cells in flow cytometry t-cell cytotoxicity assay?

New to the field of cell therapy. Why fluorescent-labeled antibodies to target cell antigens are not used for determining target cell count by flow cytometry in cytotoxicity assay (eg car-t)? Cell...

21 February 2021 3,278 3 View

How to run repeated measures in mvabund?

I have a dataset of fish species abundances collected from 6 different habitats over the course of 30 years. Fish were collected multiple times per year and environmental variables were recorded...

21 February 2021 3,838 1 View

How to calculate time-averaged fields from Ansys CFX solver?

I would appreciate any comments on the following problem. I need to calculate mean fields (e.g. pressure, velocity and vorticity), for which I activate Trn Stats tab in CFX-Pre. However, what I...

21 February 2021 8,548 7 View

Chiara Cavallini

Hi Javier, we have used both FACSCanto II (BD Biosciences) and Cytoflex (Beckman Coulter) to analyze microvescicle, but at the end we prefear FACSCanto (for the software)

Best,

Chiara

Lech Kaczmarczyk

It depends on the specific application. For routine FACS I used BD FACScanto and LSR Fortessa. Both seem to do the job well.

Ann Atzberger

Beckman Coulter's Cytoflex is optimized for microparticles, but I have not worked with it.