What is the best flow cytometer to analyze microparticles?

More Javier Crespo Asensio's questions See All

Is it possible that the Earth's axis of rotation could change?

Due to the shifting ocean currents, the planet could be thrown off balance, altering the angle of rotation, and even causing the axis to shift, plunging us into chaos. In reality, it doesn't take...

15 July 2024 8,949 1 View

Can ROBINS-I be used to measure risk of bias in single-arm studies?

I am doing a systematic review, and I am measuring risk of bias with RoB2 for RCT, and ROBINS-I for non RCT. My questions is, for single arm studies, can I use ROBINS-I? I am not sure how to...

14 July 2024 3,570 5 View

I'm looking for a tool to evaluate the risk of bias in... Are there any recommended applications or platforms?

Dear researchers, In a systematic review (not a meta-analysis) compiling studies on various diagnostic systems against a specific pathogen. The most relevant parameters evaluated are the type of...

03 July 2024 9,069 3 View

Does wind power turbine use increase world temperatures a lot and create hurricanes?

Wind turbines extract a lot of energy, about 30-35%, that passes through. Now they are building a lot of them, more and more powerful. Change wind trajectory is easy, if a lot of obstacles is in...

12 June 2024 9,002 2 View

Does variations in the number of lattice nodes affect lift and drag calculation in Lattice Boltzmann Method?

Dear colleagues, I have spent a few weeks trying to figure this problem out with no success, so that I post this question here. First of all, I have to say I am new to LBM method. I am working...

11 June 2024 1,084 3 View

How to prepare aliquots of freezing medium (for PBMC)?

Hello everyone, I am working on a project where I receive daily samples to freeze at -80 °C. Specifically they are peripheral blood mononuclear cell (PBMC), until now I was using CryoStor® CS10,...

22 April 2024 3,905 6 View

How to upload a file to Researchgate?

I'm trying to upload a recently published book to Researchgate and the response is: "An error occurred. Error parsing server response".

01 April 2024 485 2 View

Should I block compensation beads with mouse serum in flow cytometry?

I am using mouse serum to block Fc receptors before staining, for flow cytometry. My question is if I should also add the serum to compensation beads before staining them, so that the cells and...

07 March 2024 9,989 2 View

Do you can resolve geometry problem?

in the given regular triangle ABC find the locus of the points M inside this triangle such that MAB +MBC+MCA=pi/2

07 March 2024 7,514 5 View

Do you can resolve geometry problem?

In triangle ABC, the median BD is drawn from vertex B to side AC. If the length of AC is 10 cm and the length of BD is 6 cm, what is the length of the median AE drawn from vertex A to side BC?

02 March 2024 7,053 2 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

Why do we equate male and female arousal?

Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)

05 August 2024 9,537 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to make a Lateral flow control line more uniform?

We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...

31 July 2024 4,510 3 View

Does anyone know where to find polypropylene microspheres or microparticles around 1-5micrometer?

These will be used for the study of microplastics in the lung

29 July 2024 9,609 2 View

Suggestions for the Annexin V Positive Control ?

Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...

29 July 2024 8,624 1 View

Do some Staphylococcus aureus strains have in vitro antimycobacterial activity ?

Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?

29 July 2024 10,023 2 View

Why cannot i find my protein on cell surface after antibiotic selection of expressing plasmid?

I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...

28 July 2024 3,178 3 View

Problem with Two-phase simulation?

Hello; I am conducting a CFD study on two-phase flow in a separator, utilizing refrigerant as the working fluid. For this study, I have implemented pressure inlet and pressure outlet boundary...

25 July 2024 2,309 5 View

Chiara Cavallini

Hi Javier, we have used both FACSCanto II (BD Biosciences) and Cytoflex (Beckman Coulter) to analyze microvescicle, but at the end we prefear FACSCanto (for the software)

Best,

Chiara

Lech Kaczmarczyk

It depends on the specific application. For routine FACS I used BD FACScanto and LSR Fortessa. Both seem to do the job well.

Ann Atzberger

Beckman Coulter's Cytoflex is optimized for microparticles, but I have not worked with it.