Hi, I am trying to grow N.gonnorhea on chocolate agar plates and I dont seem to be getting lawns after 24 hrs of incubation at 37 degree Celsius and 5% CO2. I do not get it where I am going wrong
Use Thayer Martin media and ensure you are using sheep blood for preparation
if we are doing non-invasive sensing in patient urine then require ethical permission or not??? If we have a consent form fill with the patient then it is sufficient or not???
24 May 2024 956 0 View
My CV profile shows redox peaks at around -0.3V in the potential window -0.7 to 0.3V. But in GCD, the discharge curve is not going below -0.25V. If the potential window of GCD is followed i.e....
29 January 2024 9,836 3 View
Can we partly cure Butyl rubber sealant formulation at room temperature in 10-15 days time. The purpose of adding a curing system in the formulation is to add the tensile in the product without...
24 January 2024 3,285 3 View
Hi Can anybody help us to know how we can make zinc disorbate ?
23 January 2024 3,884 1 View
Since its multidose injection, we have benzalkonium chloride as preservative in formulation. we knew that benzalkonium chloride also act as cationic surfactant. During stability, How to avoid the...
01 January 2024 2,610 3 View
In my study I have observed that the capacitance retention rate after 1000 charging-discharging cycles increased beyond 100%. can anyone kindly provide insight in this regard. Thank...
21 December 2023 2,848 1 View
The amount of reagents to be taken
02 December 2023 3,481 0 View
procedure of preparation
19 November 2023 4,484 1 View
for monitoring disinfection byproducts in drinking water
12 October 2023 8,613 3 View
brain tumor detection and classification
22 September 2023 5,109 1 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...
06 August 2024 1,495 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View
kindly reply me. Thanking you in advance.
05 August 2024 7,727 4 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View