Dear colleages
I have a question regarding to the duration of treatment with 3MA and Rapamycin in the process of osteoblastogenesis and osteoclastogenesis.
considering this fact that the process of cell differentation (mesenchymal stem cells for osteoblastogenesis and bone marrow macrophages for osteoclastogenesis) is about 14-21 days, I want to know in which time point and for how long I have to treat the cells with RAPA/3MA.
Different articles noted different times and in some cases they did not tell about it.
I would ask if somebody has experience regarding to this issue, maybe can humbly help me.
Thanks in advance.
Azadeh
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