What is the appropriate Ligand?

More Galal Elmanfe's questions See All

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Where are the qualified researchers in management and HR?

We are looking for a research group that contains a research group in the field of management and human resources?

16 February 2024 9,102 6 View

Can I incubate my membrane with more than 1 antibody simultaneously for WB?

Aslamo alikom/ Greetings everyone, I'm conducting a western blot experiment (8% SDS gel) and I wanna test 2 proteins, one is 95 KDa, and another is 35KDa. The antibodies I've for both are mouse...

25 January 2024 7,637 2 View

Which non-linear Regression option shall I use to calculate IC50 using Graphpad prism?

Hello/Salam Everyone, I'm using GraphPad prism to calculate the IC50 of some drugs in cancer cell line and impact of drug B on the IC50s of these drugs. As per my calculation method, to conduct...

20 January 2024 7,526 0 View

What's the best solvent for Cyclophosphamide Monohydrate in cell culture?

Aslamo Alikom/Greetings everyone, I've been working on MCF7 cancer cell line, and I would like to study their response to cyclophosphamide in vitro. I was wondering what's the best solvent to...

08 November 2023 3,711 1 View

I submitted a paper, it is still under review for about 60 days. I sent the editor, her reply is under review. Should I submit to a conferencence?

I got two conferences and i think of higher acceptance rate, but I am afraid to withdraw and lose the time already taken for review.

24 July 2023 795 4 View

What can possibly cause the media to turn dark greenish?

I've been working on MCF7 cell lines for nearly 7 months now. Today while checking one of the flasks, I found its media color dark green and all cells floating dead. I've trypsinized this flask...

23 May 2023 1,373 0 View

What substitutes phenol-free media in MTT assay?

Aslamo alikom/Hello I'm planning to do MTT experiment this week but unfortunately, we ran out of phenol-free media (PFM), which is added to MTT reagent for nearly 4 hrs. As it'll take awhile to...

06 May 2023 464 3 View

Can i use seemingly unrelated equations to investigate causality among independant variables?

economertics

04 May 2023 8,512 1 View

What is the most suitable econometric model for time series analysis (27 years,11 variables)?

asked in Econometrics

28 April 2023 4,109 16 View

How to quantify polystyrene microplastic (8 micron) bioaccumulation in fish tissue?

"I have treated adult zebrafish with 8-micron polystyrene microplastic and want to study the bioaccumulation in different organs. Can this be done using hydrogen peroxide digestion followed by...

05 August 2024 853 3 View

Why doesn't chromium 2+ ion use all its d-orbitals to receive lone pairs from six waters in [Cr(H20)6)]3+?

I'm guessing it's because the ligand experiences too much electron repulsion or proton repulsion from the chromium to insert them close to the 3d-orbitals which are close to the metal nucleus. Is...

03 August 2024 1,369 1 View

Why don't d-orbitals split themselves, why does it take a ligand? why don't protons from ligand repel nucleus split d-orbitals?

why don't d-orbitals split themselves because of themselves without the presence of ligands? Electrons are indistinguishable. Why wouldn't it be more correct that protons from a ligand split the...

03 August 2024 3,588 3 View

How can you draw a coordinate bond between a metal and donor atom in metal complexes, using discovery studio visulizer software?

Hello,, The metal complex ligand appears incomplete in the screen of the discovery studio visualizer

30 July 2024 3,352 1 View

AutoGrid and Autodock 4.2.6 window opens for 1 second then closes and there is no output files?

Good day, I am a student trying to work on Autodock for a project regarding Ligand-DNA interaction so i am quite new to molecular docking. i have followed tutorials and did all the steps...

28 July 2024 2,136 4 View

Is there a method to measure the concentration of Cs, Sr, Co using spectrophotometer?

it's important to know that we are using the inactive elements of Cs, Sr, Co

25 July 2024 1,553 2 View

What does negative readings in spectrophotometer denote. Was testing TSS content of bacterial cultures?

I was performing estimation of Total Soluble Sugar content in bacterial cultures by Anthrone method. Out of 9 samples, only one sample showed positive reading while others had a negative reading...

22 July 2024 9,574 4 View

Do anyone knows any book dealing with fish growth/zootechnical performances and somatic indexes in aquaculture?

In the aquaculture and fish nutrition research field, a number of growth and somatic indexes are often used, including specific growth rate (SGR), feed conversion rate (FCR), protein efficiency...

22 July 2024 2,479 4 View

Why do fish have fishy odors naturally ?

Chemical Engineering /Food Engineering

20 July 2024 4,151 4 View

How to determine LOD values?

I am performing fluorescence experiments using a ligand to detect metal ions. I want to determine the Lowest Detection Limit (LOD) using the formula LOD = 3σ / K. However, I'm uncertain about...

19 July 2024 1,083 1 View

Alaa Frak Hussain

There are several specialized ligands doing this

Galal Elmanfe

thank you , can you give me some examples for these ligands please

There are ligands prepared to determinte lead and cadmium. For example? 1- 4-(2-pyridylazo)resorcinol. 2- 2-(4-methoxy phenyl)azo)-4,5-diphenyl imidazole

thank you for your answer