Western blot with very acidic proteins, any suggestions?

I am trying to do a Western Blot to detect acidic proteins (~30kDa) that have a PI of ~3.5, due to the presence of Asp and Glu repeats. The proteins are about 80% pure. After I ran them on...

24 January 2021 7,226 3 View

Problems with allele speration by cloning in pKS plasmid?

I am trying to do the allelic separation of a ~8 kb region using cloning strategy with pKS plasmid. However, when I analyze the result of the colony PCR sequencing It seems that both alleles were...

14 December 2020 5,829 2 View

At what concentration does hydrogen peroxide inhibit enzyme activity?

I am trying to measure GUS expression, and was wondering if anyone knew what concentration hydrogen peroxide inhibits the GUS protein??? Thanks ! Any help is appreciated!

23 November 2020 4,101 3 View

Does anyone know a Python script bank to analyze data from thermal images / thermographs?

script, phyton and thermography

13 September 2020 3,951 3 View

Management Innovation and Bahavior economic?

Hello guys! I'm looking for studies that address behavioral economics in Management innovation. Especially regarding the decision-making process.

13 September 2020 8,687 3 View

How to quantify and stratify the volume and specific surface area of submicro-, micro-, meso- and macropores in a porous solid?

Is it possible to quantify and stratify the kinds of pores (volume and specific surface area)? Only N2 physisorption is enough? Is there another technique to help this? Physically are there other...

04 August 2020 9,175 3 View

Is possible connect infrared camera - Flir One Pro 3td generation in windows or linux, to capture images and videos?

I found in internet some drivers and softwares (flironecontrol and others), If anyone knows about more softwares, please share.

29 June 2020 6,641 2 View

Dear colleagues, please lmn what volume do you use for intranasal instillation in rats?

I am in doubt as to the best volume for intranasal instillation in rats, what volume ensures the best distribution of the particles?

20 February 2019 2,920 1 View

I need to obtain an equation for calculation of pure urea in liquid phase. Has someone a refrence? Or a thermodynamic equation for this?

I'm developing a work that needs the density of pure liquid urea. It's liquid when the temperature is higher than 134 oC, but I didn't find references for this property.

29 November 2018 4,706 2 View

For CRISPR, does it matter if the guide RNA is looking for the antisense or sense strand?

So, I've used the CRISPR P 2.0 software to look up guide RNAs to use to delete a region in my gene's promoter. Past members of our lab were using one guide RNA taken straight from that website to...

01 November 2018 1,756 2 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

No title

01 March 2021 3,905 5 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Which group formation theory do you recommend for projects that aim to bring together entrepreneurs for training for regional development?

I would like to have ideas about which theories of group formation are suitable for entrepreneurial training projects, with the aim of creating bonds, building trust, and collaboration for...

28 February 2021 4,223 2 View