From a hosts point of view, which comes first, effect of toxins on pathways or identification of toxins?

From a pure biology perspective, when a pathogen affects a host, what comes first, the effect of the toxin (effector protein) on the pathways or identification of the structure of the toxin by the...

01 February 2020 8,453 6 View

How do you apply statistical testing when predicting effector proteins?

Being a computer science student, I don't know much about statistical testing. However, recently, a lot of work has reported statistical validation of their result. In machine learning-based...

10 November 2019 6,264 2 View

How to download from NCBI the FASTA of all sequences for a particular search term?

After a search term is entered, on the top right there is an option called Send to. Then click File, and click FASTA under format tab. There they show you how many entries/sequences to be...

05 June 2019 6,191 2 View

Someone help me to find one gram-positive non-pathogenic bacteria residing in human gut along with a reference claiming the same?

I want to know the names of a few species of gram-positive bacteria which are non-pathogenic and and lives in the human gut....

04 May 2019 9,236 2 View

What is the example of a gram-positive non-pathogenic bacteria?

I want to know the names of a few gram-positive non-pathogenic bacteria

04 May 2019 8,244 2 View

How do I get the corresponding nucleotide sequence of a given peptide sequence?

Suppose, I have a peptide sequence, and I want to find out the corresponding nucleotide sequence of that peptide sequence. Is there any tool that finds this out?

03 April 2019 1,752 3 View

In order to establish a new metric for quantifying something, what are the prerequisites?

Suppose I want to establish a new metric for a certain bioinformatics problem. What are the mathematical prerequisites for establishing this metric? Are lemma's and theorems required for...

01 February 2019 1,953 2 View

How does binding between 2 proteins occur?

Suppose 2 genes produce 2 proteins which would be binding. After the genes are turned on and the proteins are produced, then genes can be turned off and the proteins produced can bind with each...

01 February 2019 8,742 6 View

When a protein binds to the dna or a particular gene, does the gene need to be activated in order to bind?

So proteins bind to DNA or genes all the time. I wanted to know whether before binding with a protein, the gene needs to be activated or not. If there is a research article backing your claim,...

31 December 2018 1,180 3 View

What is the impact factor of journal of bioinformatics and computational biology (world scientific)?

Got a research gate score, but I want to know its actual impact factor. Is it a good journal in Bioinformatics?

10 November 2018 2,063 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

I have designed reflectarray element and feeding it though Horn Antenna on HFSS. After full wave simulation, I am not getting desired results?

I have designed reflectarray element to generate OAM beams at 5.8GHz and feeding it though Horn Antenna on HFSS. After full wave simulation, i am not getting desired results i.e Radiation pattern...

01 March 2021 9,701 5 View