I streaked Avian pathogenic E coli on blood agar plate. i found the results shown in picture, i am not sure what type of hemolysis is this, please guide me.
I suggest Phospholipases present in the agar media might induce lysis.
Without the area without growth demonstrating the presence of intact red blood cells, it is not clear what is going on. We should see red in the media away from the growth. E. coli normally has beta hemolysis, so this plate is somewhat confusing. Is this plate incubated for much more than 48 hrs?
Robert John Wolff it is 4h hr incubation, and it is Avian pathogenic E coli.
That is too short of a time period for the hemolysins to have diffused throughout the media. I think something else is going on.
In thinking about this I have several questions/issues:
1/ Was the agar cooled to 50 degrees C fully before adding the blood?
2/ Was the blood very fresh? The enzyme issue asked by Siva G Somasundaram might be involved and this might involve the quality of the agar and chemicals used.
3/ Did the plates with agar get heated enough to cause breakdown, but not to create chocolate agar?
4/ Did you grow controls that would show alpha and beta hemolysis to compare to you test organism?
Blood was not fresh,but i think agar was hot above 50 degree c, the plates was incubated at 37C, buti didnt grow controls , i thnk i should repeat it keep in mind these things you mentioned sir. Robert John Wolff
That would probably be best. Please let me know how it works out.
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