Do different alcohols disinfectants act on the same mechanisms to kill bacteria?

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How to remove unedited version of the manuscripts from research gate?

Our manuscript was published ahead of print. The edited and revised version is now available. How do I remove the old version of the manuscript from the research gate?

01 February 2021 5,965 1 View

How to prevent false negative FISH results?

I am analyzing data from a FISH experiment and notice that there are false negatives. What is the best option to change in the experimental set up to avoid this from happening?

04 October 2020 5,777 1 View

Clarification on Shapiro-wilk sig. , skewness and kurtosis?

Hello, Maybe is a bit silly question but, I have a variable (N=78) and on shapiro-wilk test the sig. is .008 which is just over (greater) than 0.05 meaning that is just about normally...

29 August 2020 1,384 4 View

How do you average log CFU/ml?

Do you first calculate the average of all the CFU and then sub it into the equation ? OR Do you calculate each individual log CFU/ml and then average that?

19 March 2019 7,956 6 View

What is the general upper detection limit CFU/ml for Enterococcus faecium?

How do you determine the upper detection limit for E. faecium? If there are too many to count on the plate, can I suggest that there are >25,000 CFU/ml for example? - or would that be too much

02 March 2019 1,261 1 View

How do you perform a Mann-Whitney U test when trying to calculate the average CFU/ml?

I have a treated and a control condition of agar plates. I counted the number of colonies and wanted to carry out a mann whitney test. Do I carry out the mann whitney test using the counted...

02 March 2019 4,830 1 View

How do you average log CFU/ml?

Do you first calculate the average of all the CFU and then sub it into the equation ? OR Do you calculate each individual log CFU/ml and then average that?

02 March 2019 1,412 7 View

How to find x-ray structures that have been fully solved for homology modelling?

I'm trying to use homology modelling to find the structure of the N-terminus of a protein which is thought to contribute to its hetero-oligomeric structure. However, I can't find an x-ray...

24 December 2018 6,810 3 View

What causes bacteria to have sharp/fuzzy zone of inhibitions on disc susceptibility plates?

Zone of inhibitions sometimes appear fuzzy/sharp with different antibiotic discs on the same agar plate, what are the explanations for this? Also, how important are these when determining the...

10 November 2017 8,676 0 View

Why do enzymes have higher affinity for transition state analogs compared to the substrate or product?

Why is that a higher affinity leads to increased effectiveness of a drug inhibitor? Also, most importantly why do enzymes bind more tightly to transitions state analog inhibitors? what is...

11 December 2016 2,057 3 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Why am i not getting solid after reacting APTES with salicylaldehyde?

The reaction of APTES with salicylaldehyde in ethanol leads to the formation of imine compound yellow solid which is mention in literature, but I am not getting yellow solid I don't know why? when...

28 February 2021 2,955 2 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

How to best wash a 96-well plate containing microplastic?

I am planning my protocol for my dissertation for an experiment quantifying bacterial (E. coli) biofilm formation on micro-plastic (5mm discs of polyethylene bags) by conducting a crystal violet...

23 February 2021 7,868 2 View

Are there any known solvothermal methods to synthesize MoS2 nanosheets using solvents other than water (like ethanol/ethanol-water mixture) ?

I need to synthesize MoS2 nanosheets by alternate methods without pure water as a solvent.

22 February 2021 9,246 1 View