Hi all,
I am planning to start working with HCE-2 cells and I am looking for alternatives for Keratinocyte-Serum Free Medium. I found some papers describing usage of DMEM/F12 supplemented with
10% (w/v) fetal bovine serum, 0.005 mg/ml insulin, 10ng/ml of epidermal growth factor (EGF)-penicillin G/streptomycin(5000 IU/ml,5000 μg/ml) 2mM GlutaMax-I/L-glutamine for cultivation HCE-2 cells.
Does anyone have any experience in growing HCE-2 cells in DMEM/12/supplements? Do you usually coat the flasks with Matrigel?
Thanks a lot,
The link here will help you . Good Luck!
https://link.springer.com/protocol/10.1007/978-1-62703-125-7_3
Hopefully, this article and its protocol will help you. Good luck!!
http://www.groupes.polymtl.ca/tissue/Publications/PDFs/11501.pdf
Greeting Povilas, I hope your research is going well.
I have the same doubt, I'm also planning to work with HCE-2 but in our lab we don't have the opportunity to access to serum-free products, so I'm interested to know if you have a good experience culturing these cells with DMEM and other supplements?
Thank you in advantage,
Dayan.
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