What type of Hemolysis is this?

I streaked Avian pathogenic E coli on blood agar plate. i found the results shown in picture, i am not sure what type of hemolysis is this, please guide me.

03 April 2019 7,111 9 View

Can plasmid with f1 ori, insert into m13 phage outside the cell?

I am using phagmids as a silencing technique, so there is need of co-transformation of both phagmid and m13 plasmids for core production and insertion of Phagmid into m13 phage core, to avoid...

02 March 2019 6,070 5 View

Which statistic can be apply on prevalence of HBV, HAV, Polio and Adeno viruses among different cities?

I have data of HBV, HAV, Polio and Adeno viruses prevalence among different cities? how can i interpret the results based on Odd ratio, risk factor or Mann whitney test? is there any other...

31 December 2018 6,937 5 View

How we can use Multi-locus Sequence Typing (MLST) tool for finding our desire genes?

MLST tool used for screening of housekeeping genes in a particular genome. Is this possible to search for those genes which i want to find out there presence ? I have about 30 genomes of a...

11 December 2018 8,929 4 View

How to Extract hetrophiles and macrophages from Chicken blood?

I need to know an easy protocol of Hetrophiles and Macrophages extraction from blood specially from Avian/chicken. and also want to know some related knowledge which can help me to test...

08 September 2018 7,364 4 View

How to Extract hetrophiles and macrophages from Chicken blood?

I need to know an easy protocol of Hetrophiles and Macrophages extraction from blood specially from Avian/chicken. and also want to know some related knowledge which can help me to test...

08 September 2018 4,153 0 View

Planing PhD in Molecular Microbiology any suggestions?

Hello everyone, I have almost completed my MS thesis degree. i worked on sRNA expression and silencing of virulent gene of avian Pathogenic E coli, hoping to publish my work soon. I want to pursue...

01 January 1970 6,383 6 View

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01 January 1970 2,328 7 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Do you know good and user-friendly programs to measure plant quantitative traits?

For my research i will need to measure plant quantitative traits (especially leaves area and roots length, but would be nice to add some more information). I recently discovered...

01 March 2021 5,035 2 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View