I am using sRNA to silence a gene in E coli. for expression analysis, I proceed for qPCR. so I grow the wild type and silenced type E. coli and extract RNA without adding any antibiotics except for Silenced which has antibiotic resistance gene in expression vector. dose it necessary to add Rifampicin to halt transcription at same point ? or just same OD is enough to quantify the mRNA level.
Muhammad Irfan
what should be do if there is unavailability of DNS reagent for eznymatic reaction?
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