Dear Tanmay Saha ,

Given the somewhat limited amount of information it is hard to guess.

Let me assume; you want to go HPLC (GC for highly polar components very often involves derivatization...), then you will have to find out if the component of interest is "only polar" if it is "polarizable" if it "can be ionized"...

In other words, try to specify the chemical identity of this component. Yes, if you have no other tools, use your chemical knowledge of the samples and try to use the "Sherlock Holmes"-approach. Eliminate the obvious, and try to prove that the elimination was correct.

Many times the analysis of polar components involves ion-pair chromatography, HILIC, ion exchange chromatography, etc. Depending on the physical-chemical properties of your target component(s), you will have to decide on the right detector.

UV/VIS is the most used.

Fluorescence - if feasible, often after derivatization

RI, if you use isocratic conditions, and go after high concentrations and need a universal detector

Corona CAD, if you use gradient conditions, and need a universal detector.

Then there are conductivity, amperometry, MS, MS/MS, and many other detectors available.

Why am I adding all this?

Because you will have to define your group of analytes first, and then we can help you with detailed and targeted information for your research.

I hope this was helpful.

All the very best for your research,

Detlef.

Please look at the following below links which may help you in your analysis:

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3901216/pdf/metabolites-02-00496.pdf

https://assets.thermofisher.com/TFS-Assets/CMD/Application-Notes/D22218~.pdf

https://www.avericadiscovery.com/wp-content/uploads/2016/07/NMCS-2016-Poster-EPSA.pdf

http://citeseerx.ist.psu.edu/viewdoc/download?doi=10.1.1.322.8977&rep=rep1&type=pdf

Thanks

Thanks to dear Jensen for your valuable information. But, I've already done it. I'm asking for cell line assay of highly polar compound because these are only soluble in 100% DMSO. But, I can't use 100% DMSO due to cell mortality. So, kindly give me some suggestion regarding this.

First, you need to calculate the logP for your compounds. It is easy using Chemdraw software to account for both logP and clogp.

For cell lines treatment, you need to have a stock of you compound dissolved in DMSO at conc. of 10 mM (0.01M), then you start in the first well with a conc of 100 uM, by dissolving your required volume in the media of the cells (such as DMEM, MEM, or etc..). Every cell needs special media.