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Questions related from Noor Ul Ain
I got comment on my FTIR data figure from a reviewer. The reviewer said "FTIR data in Figure should be repeated. there is no bassline." I made Y off set comparison graph of FTIR on OriginLab. Can...
04 August 2024 5,975 3 View
Hello, I want to load Remedesivir (RNA polymerase inhibitor) into mesoporous nanoparticles (MSNs). The issue is drug is temperature and pH sensitive and it degrades quickly. I need advice on its...
02 July 2024 724 3 View
Hello I have prepared metal nanoparticles and did zeta potential. Normally there is a single peak in sample but now there are 3 peaks. I prepared sample for analysis first I prepared 10 mg/mL...
01 July 2024 900 4 View
Hi I am trying to revive Pseudomonas aeruginosa from frozen glycerol stock and also from cryobeads. But there was no bacterial growth. I used nutrient agar, tryptone soy agar to streak on. Also I...
10 June 2024 6,528 9 View
Hello! I have already published an article and given all the details of protocols in the publication. Now I am working on another manuscript but basic protocols are same and already reported. How...
04 June 2024 1,131 5 View
Hi I am making FTIR graphs in percent transmittance. I observed the change in broadness and dip of the peak. So when dip increases does that mean there is a decline in the intensity of band?...
04 June 2024 1,036 3 View
I am carrying out batch adsorption studies for the removal chromium and lead. I performed an experiment with different adsorption doses 0.01, 0.05 and 0.1 g. The highest % removal was achieved at...
28 May 2024 8,516 8 View
I am performing hemolysis assay for antimicrobials but there's difficulty in selecting the concentrations of the antimicrobials. I have read the papers and MHC 90 was determined. Should I consider...
22 May 2024 8,768 3 View
Hi I have synthesized cerium oxide nanoparticles but they are not showing antibacterial activity at low dose, even at 10 mg/mL concentration there's minute antibacterial activity. Although there...
15 May 2024 3,623 4 View
Hi I have done the mechanical testing of my material. I got three results % elongation, ultimate tensile strength and modulus of elasticity represented in MPa. I observed that if % elongation of...
13 May 2024 4,523 3 View
Hello! I am doing batch adsorption studies for removal of lead. I am using lead nitrate salt for this purpose. When I adjust the initial pH of solution to 5 or above it precipitates. However, in...
07 May 2024 6,755 4 View
Hi everyone! I have done the lead adsorption studies and did the XRD of adsorbed nanocomposite. I observed new peaks in XRD diffractogram after the adsorption at 2 theta=33.01, 34.7, 37 and 40.2....
27 April 2024 9,594 3 View
I am removing the Pb(II) from wastewater using copper nanoparticles. I have done FITIR and XRD analysis after the adsorption of Pb and there is clear change in the peaks in both FTIR and XRD. In...
25 April 2024 5,022 6 View
Hi everyone! I am synthesizing cerium oxide nanoparticles using cerium nitrate hexahydrate salt. Initially when I was preparing batches yield was between 40-50%. Suppose I am taking 50 mg salt the...
22 April 2024 5,823 7 View
Hi I need help in finding a good book describing functionalized nanoclays and their biomedical applications. I have been looking for it over google scholar but unable to find the research, review...
17 April 2024 5,970 1 View
I have deacetylated chitosan using 50% w/w NaOH for 6 hours. But still it doesn't dissolve completely. Should I repeat the deacetylation step? Thank you for your guidance.
18 March 2024 1,967 5 View
The hydrogels I have prepared are most like thin membranous sheets. I want to carry out full-thickness wound animal (male rat) studies (6 mm diameter). Should I cut the hydrogels also 6 mm or...
19 October 2023 9,374 3 View
Hi I want to check the cell proliferation using live/dead cell imaging technique till 72 hr. What should be the cell seeding at the start if using 24 well plate. Thanks for your time.
04 October 2023 7,691 9 View
I want to assess the cell migration rate through scratch wound assay for the hydrogel. I have read the literature and in some research articles it is mentioned that DMEM media was not supplemented...
26 September 2023 4,453 9 View
Hi I have done the removal of heavy metals from the aqueous solutions through batch experiments. I observed that with increasing the concentration adsorbent (g) the % removal of heavy metal has...
18 September 2023 559 17 View
Hi! I want to perform the MTT assay to assess the cytotoxicity of my hydrogels. I have read literature. It is reported that either use the extract of the hydrogels by dipping them into the DMEM...
14 August 2023 1,663 2 View
I want to check the cytotoxic effect of my hydrogels. Before conducting the experiment, in literature it has been reported that to wash the hydrogels with ethanol and then PBS thrice to remove the...
07 July 2023 6,039 1 View
Hi, I am carrying out the MTT assay using BJ-5ta fibroblasts cell line to evaluate the cytotoxicity of the nanocomposite hydrogels. I want guidance about deciding the controls in the...
05 July 2023 9,143 4 View
I want to check the cytotoxicity of my material. I am using MTT assay kit. The protocol is provided in the booklet with kit how to perform the assay. Is it better to follow the protocol provided...
04 July 2023 2,802 4 View
I want to perform MTT assay for BJ-5ta and HaCat cell lines. I want to know before performing the actual experiment how many passages should be done of the cell lines?
27 June 2023 5,402 3 View
I want to perform the MTT assay for nanocomposite hydrogels against fibroblasts and keratinocytes. There are two methods reported in the literature; direct and in direct. Direct involves the...
22 June 2023 1,025 4 View
I want to carry out MTT assay for nanocomposite hydrogel against fibroblasts and keratinocytes. How to calculate the number of cells seeded for each cell line?
22 June 2023 816 10 View
Paclitaxel is a hydrophobic drug therefore I dissolved it in DMSO. The issue with DMSO is it has an OD value around 260 nm and the lambda max of paclitaxel is also 263 nm. Stock solution of drug...
11 May 2023 9,710 4 View
I have done the characterization-particle size of metal nanoparticles. The PDI value ranges of from 0-1. I want to know which PDI value is considered the best either closer to 0 or close to 1. In...
18 April 2023 3,330 5 View
I want to quantify the chromium in solution. I want to know whether atomic adsorption spectroscopy is better or UV vis spectrophotometer.
10 April 2023 2,363 16 View
I prepare a chitosan solution in acetic acid (0.5 M). Some of the small threads are remained in the solution. In literature it is reported that solution is filtered before using. I want to know...
17 March 2023 8,611 4 View
Hi I am using copper nitrate salt for my experiments. After 2 years, the salt crystals turned into liquid. I am using copper nitrate trihydrate salt. Kindly let me know how can i store copper...
16 March 2023 2,462 5 View
I have synthesized folate grafted PHB nanoparticles and its zeta potential has become positive. Does it imply that nanoparticles are stable?
09 March 2023 7,163 2 View
In vitro release of drug loaded PHB nanoparticles was carried out at pH 4. There is a burst release of drug at acidic pH as compared to the neutral pH. Does the crystallinity of PHB plays role in...
09 March 2023 5,602 1 View
I am preparing chitosan film by casting chitosan solution into petri dish and let it air dry. I am using NaOH to peel off the dried film but it breaks the film. I have used different molar...
08 March 2023 8,103 4 View
I have powdered diphenylcarbazide and I want to make 0.5% w/v solution of diphenyl carbazide. Since it is dissolved in acetone. Does it mean 0.5 g in 100 mL acetone? Is there any need to add...
08 February 2023 9,095 7 View
I want to deacetylate the chitosan in sodium hydroxide (50 % w/w) solution. Does that mean 50 g NaOH in 50 mL of distilled water? What should be the appropriate time and temperature for the...
08 February 2023 2,202 3 View
I have prepared the material for the removal of heavy metals from the wastewater. Now, I want to test it over the filter. How can I coat the material on the filter? Either should I dip the filter...
26 January 2023 8,659 5 View
Hello I have received the FTIR graph after the analysis of the sample but the graph isn't aligned to the baseline. I am attaching the file. Kindly guide me is it the sample or machine error?
20 January 2023 3,166 15 View
I have synthesized a nanocomposite membrane for heavy metals removal like Cr(VI) and Pb(II). I want to perform batch adsorption experiment. Kindly suggest me the appropriate time period for the...
13 November 2022 2,114 5 View
Hi, I have recently installed origin 2022 pro version. I want to make overlapping FTIR graphs in it. But I am unable to move the lines of the graph. Kindly help. Thanks
17 October 2022 6,698 4 View
Chitosan is a natural biopolymer with antibacterial properties and the solution of chitosan is prepared in acetic acid. So, has acetic acid any effect in contributing towards the antibacterial...
20 May 2022 584 4 View
I have synthesized cerium oxide nanoparticles via hydrolysis method using NaOH. I obtained yellow precipitates at the end of reaction indicating synthesis of cerium oxide nanoparticles. But in UV...
28 March 2022 9,279 26 View
I have prepared the silver nanoparticles via chemical reduction method using NaBH4. Upon turning yellow color of the solution I stopped the reaction. I got UV vis peak of nanoparticle solution at...
15 March 2022 7,458 14 View
I want to analyze the bacterial cell membrane damage via TEM. For the preparation of sample of TEM which chemicals are used? I have read the literature and there are different chemicals. After...
21 January 2022 9,236 8 View
Hi everyone, I have synthesized silver nanoparticles through chemical reduction method, instead brown or yellow i obtained green color. I checked the UV-vis peak of the nanoparticles it is around...
18 November 2021 9,347 3 View
Hi I am working on removal of heavy metals from the wastewater using nanocomposite membranes. Although the heavy metal removal is above 90% but adsorption capacity is low. Can someone please guide...
20 September 2021 7,310 13 View
Hello everyone! I am using soxhlet for determination of gel fraction of the nanocomposite films. While operating soxhlet bubbles are formed in the column and cycle doesn't run completely. I need...
25 August 2021 5,854 2 View
Hi! I am working on polymer nanocomposite membranes and their physical properties. Is it possible that metal nanoparticles enhance the swelling property of polymer but decrease the porosity? Or...
19 August 2021 5,901 6 View
I want to evaluate the antibacterial activity of metal nanoparticles at different time intervals. I am performing this experiment in a flask and after the specified time take out the sample for...
13 August 2021 4,275 5 View
I am performing Pb batch adsorption experiments. While preparing the working solution and adjusting the pH of Pb solution, the solution precipitates at pH above 4. Another issue that i am facing...
13 August 2021 6,680 8 View
