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Questions related from Noor Ul Ain
Hi! I want to perform the MTT assay to assess the cytotoxicity of my hydrogels. I have read literature. It is reported that either use the extract of the hydrogels by dipping them into the DMEM...
14 August 2023 1,639 2 View
I want to check the cytotoxic effect of my hydrogels. Before conducting the experiment, in literature it has been reported that to wash the hydrogels with ethanol and then PBS thrice to remove the...
07 July 2023 6,026 1 View
Hi, I am carrying out the MTT assay using BJ-5ta fibroblasts cell line to evaluate the cytotoxicity of the nanocomposite hydrogels. I want guidance about deciding the controls in the...
05 July 2023 9,132 4 View
I want to check the cytotoxicity of my material. I am using MTT assay kit. The protocol is provided in the booklet with kit how to perform the assay. Is it better to follow the protocol provided...
04 July 2023 2,794 4 View
I want to perform MTT assay for BJ-5ta and HaCat cell lines. I want to know before performing the actual experiment how many passages should be done of the cell lines?
27 June 2023 5,389 3 View
Paclitaxel is a hydrophobic drug therefore I dissolved it in DMSO. The issue with DMSO is it has an OD value around 260 nm and the lambda max of paclitaxel is also 263 nm. Stock solution of drug...
11 May 2023 9,698 4 View
I have done the characterization-particle size of metal nanoparticles. The PDI value ranges of from 0-1. I want to know which PDI value is considered the best either closer to 0 or close to 1. In...
18 April 2023 3,319 5 View
I want to quantify the chromium in solution. I want to know whether atomic adsorption spectroscopy is better or UV vis spectrophotometer.
10 April 2023 2,354 16 View
I prepare a chitosan solution in acetic acid (0.5 M). Some of the small threads are remained in the solution. In literature it is reported that solution is filtered before using. I want to know...
17 March 2023 8,601 4 View
Hi I am using copper nitrate salt for my experiments. After 2 years, the salt crystals turned into liquid. I am using copper nitrate trihydrate salt. Kindly let me know how can i store copper...
16 March 2023 2,450 5 View
I have synthesized folate grafted PHB nanoparticles and its zeta potential has become positive. Does it imply that nanoparticles are stable?
09 March 2023 7,147 2 View
I am preparing chitosan film by casting chitosan solution into petri dish and let it air dry. I am using NaOH to peel off the dried film but it breaks the film. I have used different molar...
08 March 2023 8,096 4 View
I have prepared the material for the removal of heavy metals from the wastewater. Now, I want to test it over the filter. How can I coat the material on the filter? Either should I dip the filter...
26 January 2023 8,613 5 View
Hello I have received the FTIR graph after the analysis of the sample but the graph isn't aligned to the baseline. I am attaching the file. Kindly guide me is it the sample or machine error?
20 January 2023 3,152 15 View
Chitosan is a natural biopolymer with antibacterial properties and the solution of chitosan is prepared in acetic acid. So, has acetic acid any effect in contributing towards the antibacterial...
20 May 2022 571 4 View
I have synthesized cerium oxide nanoparticles via hydrolysis method using NaOH. I obtained yellow precipitates at the end of reaction indicating synthesis of cerium oxide nanoparticles. But in UV...
28 March 2022 9,267 26 View
I have prepared the silver nanoparticles via chemical reduction method using NaBH4. Upon turning yellow color of the solution I stopped the reaction. I got UV vis peak of nanoparticle solution at...
15 March 2022 7,444 14 View
I want to analyze the bacterial cell membrane damage via TEM. For the preparation of sample of TEM which chemicals are used? I have read the literature and there are different chemicals. After...
21 January 2022 9,225 8 View
Hi everyone, I have synthesized silver nanoparticles through chemical reduction method, instead brown or yellow i obtained green color. I checked the UV-vis peak of the nanoparticles it is around...
18 November 2021 9,335 3 View
Hi I am working on removal of heavy metals from the wastewater using nanocomposite membranes. Although the heavy metal removal is above 90% but adsorption capacity is low. Can someone please guide...
20 September 2021 7,302 13 View
Hello everyone! I am using soxhlet for determination of gel fraction of the nanocomposite films. While operating soxhlet bubbles are formed in the column and cycle doesn't run completely. I need...
25 August 2021 5,846 2 View
Hi! I am working on polymer nanocomposite membranes and their physical properties. Is it possible that metal nanoparticles enhance the swelling property of polymer but decrease the porosity? Or...
19 August 2021 5,891 6 View
I want to evaluate the antibacterial activity of metal nanoparticles at different time intervals. I am performing this experiment in a flask and after the specified time take out the sample for...
13 August 2021 4,260 5 View
I am performing Pb batch adsorption experiments. While preparing the working solution and adjusting the pH of Pb solution, the solution precipitates at pH above 4. Another issue that i am facing...
13 August 2021 6,672 8 View
