Paclitaxel is a hydrophobic drug therefore I dissolved it in DMSO. The issue with DMSO is it has an OD value around 260 nm and the lambda max of paclitaxel is also 263 nm. Stock solution of drug is prepared in DMSO by dissolving 10 mg/mL and further 2 ug/mL of drug dilution was prepared in DMSO as well but OD value is 1.5. I need assistance how to cut the value of DMSO so I can find out the actual peak of drug and then concentration.
Thank you for your help!
Did you try using diluted DMSO. And does drug precipitate out if it is diluted in water.
Blanking out didnt work?
Dear, Noor Ul Ain
There is no way to get spectrum of paclitaxel if it recorded in DMSO. Note, that extinction coefficient and concentration can be calculated if spectrum recorded in transparent solvent.
Iaroslav Kuziv Since paclitaxel is hydrophobic, I used DMSO to dissolve it.
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