I want to evaluate the antibacterial activity of metal nanoparticles at different time intervals. I am performing this experiment in a flask and after the specified time take out the sample for UV-vis. I am using PBS as growth media for bacteria. At 6 h the OD declines. What could be the possible reason of this? Also what should be the appropriate volume of the media?
PBS is not a good medium for the bacteria to grow. I would suggest using an appropriate growth medium for the type of bacteria you are using. Also, the no drug control should show growth which would indicate the medium you are using for the bacteria is right.
Thank you so much for the kind responses. I will take into consideration, instead using PBS would use a appropriate growth media. Sharmada Swaminath Phil Geis
Does OD drops at 6 hour in reference sample (sample without bacteria if u have such reference)?
Also, if it is not, what microorganism are you using and does OD drops in reference sample of PBS and bacteria Without nanoparticles?
Viktors Vibornijs my reference is PBS without bacteria. I am using E. coli for the experiment.
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