Hello
I have received the FTIR graph after the analysis of the sample but the graph isn't aligned to the baseline. I am attaching the file. Kindly guide me is it the sample or machine error?
possibly a machine error, but it can be corrected by any software, like using origin and doing baseline correction.
Noor Ul Ain
- Run a standard material
- Contact the manufacturer or distributor/agent with the above
It's a machine error, but it can be corrected by any software, like using origin and doing baseline correction.
I am not sure, however, this link might be useful:
https://www.shimadzu.com/an/service-support/faq/ftir/5/index.html
(A : The IR spectrum baseline may be curved due to the reasons below.
(1) Effects of scattering (transmission method, specular reflection method, etc.)
The baseline of the transmission spectrum may drop off at the left (%T display) if the sample has a rough surface or contains inorganic compound powder. This is caused by scattering of the infrared light at the sample surface or interior, and this effect is greater for the shorter wavelength. Consequently, an IR spectrum affected by scattering drops more (%T display) towards the short wavelength (high wavenumber) end.
The diagram at the right shows the microscope transmission spectrum of magnesium stearate that drops off to the left due to the effects of scattering.)
Yaseen Ayub I tried to correct it on origin but it is still not at the same level. Can you please guide me?
Yaseen Ayub thanks for sharing the video, i followed the same tutorial but as you can see the FTIR graph of my sample is quite elevated in fingerprint region so when i try to adjust it, it cuts either the beginning region or fingerprint
Have you ever try to check lamp energy, yet? After that can you repeat run on the PS sheet or any standard material or reference material to check the performance of the instrument, first?
Thiphol Satarpai basically I sent my samples for the analysis to the technician. He performed the analysis. He doesn't know much details. He processed sample from 400 - 4000 cm-1 with scan speed 4 cm-1.
It is depend on sample-preparation and instrument skill. So sorry, I can't help.
Hello,
Is it possible to edit the base line of your FT-IR graph using Origine software, besides you can smooth and change a lot things.
For more details try to follow this link and watch this video steps.
https://www.youtube.com/watch?v=fCnUUUPpHGE
Good luck
Better to adjust it with Origin.
There are a number of potential causes for a baseline error in FTIR spectroscopy. Some common causes include:
- Improper instrument alignment or calibration: Before running a sample, it is important to ensure that the instrument is properly aligned and calibrated. This includes ensuring that the beamsplitter, detector, and reference mirror are properly adjusted, and that the instrument is zeroed correctly.
- Sample preparation issues: The sample must be prepared correctly, such as ensuring that it is homogenous and free of any contaminants that might interfere with the analysis. The sample should also be placed on the ATR crystal in a consistent and reproducible manner.
- Background noise: Interference from ambient light, vibration, or other sources of noise can cause baseline errors in FTIR spectra.
- Data processing errors: Improper data processing techniques, such as using the wrong baseline correction algorithm or incorrect integration parameters, can also lead to baseline errors.
It's hard to tell with the information provided. It might be a sample or machine error. It is advisable to check the instrument alignments and sample preparation again and repeat the measurement. If the problem persists, it may be necessary to consult the manufacturer or an expert for further assistance.
The reasons for the baseline error in FTIR spectroscopy can be multifaceted and may include:
To minimize the baseline error in FTIR spectroscopy, it is important to optimize the instrument settings, ensure proper sample preparation and handling, and apply appropriate baseline correction methods. By doing so, one can obtain accurate and reliable spectral data for chemical analysis and characterization.
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