Hi,
I am carrying out the MTT assay using BJ-5ta fibroblasts cell line to evaluate the cytotoxicity of the nanocomposite hydrogels. I want guidance about deciding the controls in the experiment.
blank: media+MTT solution (to cancel the background noise)
positive control: Cells+media+MTT solution
control hydrogel (containing no nanoparticles)
hydrogels (containing different concentrations of the nanoparticles)
negative control : here I am confused and need guidance, whether it should be DMSO+cells+MTT solution
I will be grateful for your help!