Hi,

I am carrying out the MTT assay using BJ-5ta fibroblasts cell line to evaluate the cytotoxicity of the nanocomposite hydrogels. I want guidance about deciding the controls in the experiment.

blank: media+MTT solution (to cancel the background noise)

positive control: Cells+media+MTT solution

control hydrogel (containing no nanoparticles)

hydrogels (containing different concentrations of the nanoparticles)

negative control : here I am confused and need guidance, whether it should be DMSO+cells+MTT solution

I will be grateful for your help!

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