Loading Remdesivir into mesoporous nanoparticles involves several steps, ensuring the drug is effectively encapsulated and released in a controlled manner. Here is a general procedure:

Materials Needed:

• Remdesivir
• Mesoporous nanoparticles (e.g., silica nanoparticles)
• Solvent (e.g., ethanol or water)
• Ultrasonicator
• Centrifuge

Procedure:

Notes:

• Optimize the drug-to-nanoparticle ratio to maximize loading efficiency.
• Ensure uniform dispersion of nanoparticles during the mixing process.
• Conduct stability studies to ensure the drug remains stable within the nanoparticles.

This procedure can be tailored based on specific experimental conditions and desired outcomes.

Idowu Goodnews Oluwaseyi Thanks for your detailed guidance about loading. Another problem that I am facing that this drug degrades quickly. I am concerned about its release as it is temperature-sensitive, even if I keep the dilution of drug (not loaded into MSNs) in the refrigerator at 4 degrees next day I take a UV vis spectral scan there is no peak of drug.

Apparently, the inclusion of Remdesivir in mesoporous nanoparticles, as such or at the stage of their synthesis. is not good idea, due to the instability of R and, possibly, its low bioavailability. Microencapsulation by complex coacervation, with possible involving CD, may be more frutful approach. Good luck!