I want to perform the MTT assay for nanocomposite hydrogels against fibroblasts and keratinocytes. There are two methods reported in the literature; direct and in direct. Direct involves the direct seeding of cells on the hydrogels whereas indirect involves the use of extracts of the hydrogel. Please guide me which method is better
The answer relies in the nanocomposite, some ones interfere with the mtt assay giving false positive results. In those cases you can use a soluble mixture that you can wash before apply the mtt
Jahaziel Gasperin Bulbarela how would i know whether its interfering with the mtt assay or not? Is it good I apply both direct and indirect method?
It is by trial and error, if direct method with proper controls, like dmso 5-10%, dosent give reliable results, you use the pther metod
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