33 Questions 22 Answers 0 Followers
Questions related from Muhammad Nur Hasan
Previously, I made Pb(NO3) stock in miliQ water and diluted into buffer. But I tried different way yesterday. I prepared 100 uM of Pb(NO3) in buffer 10 mM TA containing 50 mM NaCl, pH: 7.0-7.4 The...
02 February 2020 1,592 2 View
Yesterday I synthesized 13 nm colloidal gold nanoparticles using hotplate stirrer (T= 300 C, s= 600 rpm), the solution boiled for a long time (until bubles out). Even it had already ~ more than 1...
04 April 2019 612 6 View
Normally, I have fresh prepared TAE/TBE buffer. But sometimes I see one of my colleagues who re-use it max. 3 times, Is it corret or not? Does it have any effect to the result? Thanks
03 March 2019 757 3 View
I have DNA (disulfide modifier) has already become the Thiol due to adding TCEP. So I want to make it be disulfide again. Thanks
08 August 2018 424 0 View
I need to block AuNPs surface (space between Thiol DNA - AunNPs) to prevent binding interaction with streptavidin.
08 August 2018 6,670 2 View
I have 25 mM of Tris-acetate pH = 7.4 But, I need 25 mM of Tris-acetate pH = 8.2 for washing my DNA. I read a protocol, the adjuster should be using Acetate glacial. But, I have already adjusted...
08 August 2018 8,018 3 View
Yesterday I prepared 100 mM of MgCl in PBS 1X then sterilized it by autoclave. But my buffer was precipitated. How to prevent this matter? Thanks
08 August 2018 3,881 0 View
I read one paper (Hai-Bo Wang, 2013/DOI: 10.1039/c3nj00328k), he claim that there are approximately 50 strands of DNA probe for each AuNP. But, when I check a table from this paper (please check...
07 July 2018 1,329 1 View
I want to check the DNA strand displacement possibility of my DNA.
06 June 2018 605 0 View
I use that DNA marker in same concentration of gel (10%) and running at 80 V for 80 minutes (everything is the same).
05 May 2018 8,009 8 View
My DNA is sensitive with mechanical force such as vortex and spin-down machine. Thanks
05 May 2018 817 3 View
I thought that Thiol (-SH) is positive charge, because it can bind to metal (eg. AuNPs) that has been coated by citric acid that would be negative.
05 May 2018 7,827 0 View
When I checked (making sure) citrate-AuNPs and Streptavidin be able to bind using my own step: 1. I made line in nitrocellulose membrane by 1 mg/mL of streptavidin. 2. I droped citrate-capped gold...
05 May 2018 3,648 0 View
I have experience making EDTA solution before. And I got success when I adjust pH to 8.0 (normally many references suggest this way). But, I can not dissolve it now. What's the problem? Does...
03 March 2018 2,040 10 View
I stored Tris solution for one night after preparation. Then it changed to half the crystals.
03 March 2018 9,336 0 View
I would check my DNA (length: 80 bp and 23 bp) using PAGE (polyacrylamide gel electrophoresis). The conditions are following these: Fig 1. is 10 % gel, 100 V, 70 minutes Fig 2. is 10 % gel, 100 V,...
02 February 2018 1,844 0 View
I can't open NCBI website http://www.ncbi.nlm.nih.gov/
02 February 2018 1,846 0 View
Normally, when we prepare TAE 1X from 50X or 10X, it will contain 1 mM of EDTA in the final concentration. (http://www.bio-rad.com/webroot/web/pdf/lsr/literature/Bulletin_6205.pdf) But, I want to...
02 February 2018 5,264 0 View
When I synthesized AuNPs, I set the temperature of hotplate to 100 degrees C (according this...
02 February 2018 2,880 3 View
I want to chelate metal ion using EDTA. I saw in blood tube for anti-coagulant, they use K2-EDTA. But I have disodium salt EDTA in my lab (mw: 372.24 g/mol). So, which one is better? Thank you.
02 February 2018 6,273 3 View
The red color turns to purple.
01 January 2018 5,110 0 View
I have buffer was stored more than 6 months. Sometime the pH is changed. Suppose, what causes of the pH can be changed in the stored buffer? Thanks.
01 January 2018 6,736 3 View
I have buffer containing sucrose solution for pre-treatment and washing. When I sterilized it in autoclave, sometimes the color will be changed (almost brown).
01 January 2018 9,046 2 View
Tm Calculator websites: 1. https://sg.idtdna.com/calc/analyzer 2. https://www.biophp.org/minitools/melting_temperature/demo.php?formula=basic 3....
12 December 2017 3,413 5 View
When I made 10% SDS (w/v), it was difficult to dissolve. The powder were still staying in solution (milliQ water 100 ml). Eventhough I mixed by stirrer and heat up at 25 C. After that, I sonicated...
12 December 2017 9,884 5 View
I would like to make those buffers.
10 October 2017 5,121 0 View
Which one the most correct, I read two protocols:1. Dissolve Tris-base and NaCl in acetate acidor2. Dissolve Tris-base and NaCl in DI water, but adjust the pH using acetate acid (Not HCl).
09 September 2017 3,518 3 View
In my case, They should bind to test line (biotin-DNA) only, not bind to the control line (streptavidin).
09 September 2017 1,555 0 View
I have problem in my thesis. I have read some papers and found the explanation that Transmission Electron Microscopy (TEM) is the most common technique for obtaining accurate data about the...
02 February 2017 2,833 4 View
The 8–17 DNAzyme reaction. In the presence of Pb2+, the 17E enzyme catalyzes the cleavage of the substrate (rA).
01 January 2017 9,629 5 View
I ajusted my MgCl2 buffer to get pH from 6.5 to 7.2 by 10% NaOH. But, the color of my solution be changed to cloudy (see at the pictures). What's happened?
01 January 1970 5,977 3 View
In that case, as well as the effect of added 8 % sucrose solution. I'm gratefull if somebody help me. Thank you
01 January 1970 7,257 1 View
I have 1 mM AuNPs solution. Then I mixed with buffer (ration 1:1) Tube 1 --> Tris-HCl EDTA (TE 1X) Tube 2 --> 10 mM Tris-acetate containing 50 mM NaCl The color is change to purple (the...
01 January 1970 8,855 2 View
