Actually how many the limitations of TAE or TBE to use (re-use) in gel electrophoresis?

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How much degree Celcius for boiling HAuCl4 solution?

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How to solve precipitated PBS; 100 mM MgCl ?

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Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

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Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

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How optimize the Size Exclusion Chromatography?

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Why are my bands weird in this Agarose gel?

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What do I do with DLS peaks that are from the buffer?

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Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

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What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

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Ma Dejun

We did not see any significant effect to the result within five times. If the electric current exceeds 75 mA at 130 V, it is necessary to change the buffer.

Roba Alatawy

You can use your buffer several times as long as you don't see obvious contamination it has no effect on your gel results.

Alejandro Martin

I reuse it quite a bit; two/three times is fine. Just make sure you recirculate the buffer, or else most EtBr will migrate towards the cathode and you will get unevenly stained gels or worse, a drop in sensitivity. Also, make sure the gels are prepared with the same batch of electrophoresis buffer.

Hope it helps!