Adjusting pH of 100 mM MgCl2

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Rob Keller

Dear Muhammad,

By adding NaOH you create magnesiumhydroxide (Mg(OH)2). This is a insoluble salt in water (just check the solubility tables of salts you can find anywhere on the web).

You don't mention which buffer system you use but such a subtle pH change can easily be achieved by changing the ratio of the acid and its conjugated base.

Hope this solves the mystery for you.

Achim Recktenwald

is your NaOH perhaps contaminated with phosphates? If I remember correctly, Mg-phosphates precipitate. However, this yellow color in your liquid is a bit strange.

I would try again starting from fresh MgCl2 but also fresh NaOH-solution. And make sure the glassware etc. is properly clean.

Muhammad Nur Hasan

OK. I would like to prepare the new one.

Thank you for your suggestions, Rob Keller and Achim Recktenwald