I can't open NCBI website http://www.ncbi.nlm.nih.gov/
Previously, I made Pb(NO3) stock in miliQ water and diluted into buffer. But I tried different way yesterday. I prepared 100 uM of Pb(NO3) in buffer 10 mM TA containing 50 mM NaCl, pH: 7.0-7.4 The...
01 February 2020 1,521 2 View
Yesterday I synthesized 13 nm colloidal gold nanoparticles using hotplate stirrer (T= 300 C, s= 600 rpm), the solution boiled for a long time (until bubles out). Even it had already ~ more than 1...
03 April 2019 524 6 View
Normally, I have fresh prepared TAE/TBE buffer. But sometimes I see one of my colleagues who re-use it max. 3 times, Is it corret or not? Does it have any effect to the result? Thanks
02 March 2019 674 3 View
I have DNA (disulfide modifier) has already become the Thiol due to adding TCEP. So I want to make it be disulfide again. Thanks
07 August 2018 318 0 View
I have 25 mM of Tris-acetate pH = 7.4 But, I need 25 mM of Tris-acetate pH = 8.2 for washing my DNA. I read a protocol, the adjuster should be using Acetate glacial. But, I have already adjusted...
07 August 2018 7,940 3 View
I need to block AuNPs surface (space between Thiol DNA - AunNPs) to prevent binding interaction with streptavidin.
07 August 2018 6,595 2 View
Yesterday I prepared 100 mM of MgCl in PBS 1X then sterilized it by autoclave. But my buffer was precipitated. How to prevent this matter? Thanks
07 August 2018 3,736 0 View
I read one paper (Hai-Bo Wang, 2013/DOI: 10.1039/c3nj00328k), he claim that there are approximately 50 strands of DNA probe for each AuNP. But, when I check a table from this paper (please check...
06 July 2018 1,222 1 View
I want to check the DNA strand displacement possibility of my DNA.
05 June 2018 531 0 View
I use that DNA marker in same concentration of gel (10%) and running at 80 V for 80 minutes (everything is the same).
04 May 2018 7,935 8 View
I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.
02 March 2021 7,735 3 View
For my research i will need to measure plant quantitative traits (especially leaves area and roots length, but would be nice to add some more information). I recently discovered...
01 March 2021 5,035 2 View
I have found only four of them; www.surveyplanet.com www.online-anket.gen.tr www.surveey.com and of course google forms. Expecting to see your suggestions if there is any. Thank you
28 February 2021 3,237 3 View
I would like to use some photo from a government website for my questionnaire. But, different photo is from the different owner. So, should I just get permission from that government body or still...
23 February 2021 1,631 3 View
I am looking for hsp90 mRNA sequence in Hela and U2OS cell lines, I have tried through ncbi but always the output is hsp90 in homo sapiens not in the specific cell line. Kindly, could any one...
22 February 2021 1,853 3 View
I treid to use Entrez Batch, but it redirects me to NCBI site. Is there any script of Linux or Python through which I can download Fasta sequence of list of gene ID ?
21 February 2021 7,783 12 View
Please could someone recommend a resource that explains performing meta-regression with multiple moderators. I have read the STATA META ANALYSIS REFERENCE MANUAL, but this only gives examples of...
17 February 2021 7,376 1 View
I want to create .abs file ( absorption) for simulating solar cell in SCAPS. Can anyone pls provide me information regarding the libraries (websites) where from i can get the absorption data of...
10 February 2021 6,034 7 View
G6PD deficiency is quite common in our anesthesia practice in the middle east. Intraop/postop analgesia in them is an issue here. Many websites/books give contrasting advise with former advocating...
10 February 2021 4,817 3 View
Up to what extent website personalization can help in creating urge to buy impulsively? The digital transformation is making most of the organizations to go online and start doing business through...
09 February 2021 4,430 6 View
