Why did my lead nitrate solution precipitate?

Previously, I made Pb(NO3) stock in miliQ water and diluted into buffer. But I tried different way yesterday. I prepared 100 uM of Pb(NO3) in buffer 10 mM TA containing 50 mM NaCl, pH: 7.0-7.4 The...

01 February 2020 1,521 2 View

How much degree Celcius for boiling HAuCl4 solution?

Yesterday I synthesized 13 nm colloidal gold nanoparticles using hotplate stirrer (T= 300 C, s= 600 rpm), the solution boiled for a long time (until bubles out). Even it had already ~ more than 1...

03 April 2019 524 6 View

Actually how many the limitations of TAE or TBE to use (re-use) in gel electrophoresis?

Normally, I have fresh prepared TAE/TBE buffer. But sometimes I see one of my colleagues who re-use it max. 3 times, Is it corret or not? Does it have any effect to the result? Thanks

02 March 2019 674 3 View

How to make disulfide from thiol?

I have DNA (disulfide modifier) has already become the Thiol due to adding TCEP. So I want to make it be disulfide again. Thanks

07 August 2018 318 0 View

Whether acetic acid (acetate glacial) can be replaced with hydrochloride (HCl) to reduce pH?

I have 25 mM of Tris-acetate pH = 7.4 But, I need 25 mM of Tris-acetate pH = 8.2 for washing my DNA. I read a protocol, the adjuster should be using Acetate glacial. But, I have already adjusted...

07 August 2018 7,940 3 View

How to solve precipitated PBS; 100 mM MgCl ?

Yesterday I prepared 100 mM of MgCl in PBS 1X then sterilized it by autoclave. But my buffer was precipitated. How to prevent this matter? Thanks

07 August 2018 3,736 0 View

How to calculate the amount of DNA strands for each goldnanoparticle?

I read one paper (Hai-Bo Wang, 2013/DOI: 10.1039/c3nj00328k), he claim that there are approximately 50 strands of DNA probe for each AuNP. But, when I check a table from this paper (please check...

06 July 2018 1,222 1 View

How much melting temperature and delta G are used by benchmarks to avoid DNA strand displacement?

I want to check the DNA strand displacement possibility of my DNA.

05 June 2018 531 0 View

Why the results of my marker (DNA Ladder 50 bp) bands turn to worse?

I use that DNA marker in same concentration of gel (10%) and running at 80 V for 80 minutes (everything is the same).

04 May 2018 7,935 8 View

How to move the DNA down that attached at the tube wall?

My DNA is sensitive with mechanical force such as vortex and spin-down machine. Thanks

04 May 2018 752 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

What might be the reason for obtaining two bands in plant genomic DNA extraction?

I got a thin band and a thick band i guess it would be the genomic DNA and the 260/280 ratio is 3.

21 February 2021 6,523 6 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

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17 February 2021 4,217 3 View