Why did my lead nitrate solution precipitate?

Previously, I made Pb(NO3) stock in miliQ water and diluted into buffer. But I tried different way yesterday. I prepared 100 uM of Pb(NO3) in buffer 10 mM TA containing 50 mM NaCl, pH: 7.0-7.4 The...

01 February 2020 1,521 2 View

How much degree Celcius for boiling HAuCl4 solution?

Yesterday I synthesized 13 nm colloidal gold nanoparticles using hotplate stirrer (T= 300 C, s= 600 rpm), the solution boiled for a long time (until bubles out). Even it had already ~ more than 1...

03 April 2019 524 6 View

Actually how many the limitations of TAE or TBE to use (re-use) in gel electrophoresis?

Normally, I have fresh prepared TAE/TBE buffer. But sometimes I see one of my colleagues who re-use it max. 3 times, Is it corret or not? Does it have any effect to the result? Thanks

02 March 2019 674 3 View

How to make disulfide from thiol?

I have DNA (disulfide modifier) has already become the Thiol due to adding TCEP. So I want to make it be disulfide again. Thanks

07 August 2018 318 0 View

Whether acetic acid (acetate glacial) can be replaced with hydrochloride (HCl) to reduce pH?

I have 25 mM of Tris-acetate pH = 7.4 But, I need 25 mM of Tris-acetate pH = 8.2 for washing my DNA. I read a protocol, the adjuster should be using Acetate glacial. But, I have already adjusted...

07 August 2018 7,940 3 View

What is good idea to block AuNPs surface?

I need to block AuNPs surface (space between Thiol DNA - AunNPs) to prevent binding interaction with streptavidin.

07 August 2018 6,595 2 View

How to solve precipitated PBS; 100 mM MgCl ?

Yesterday I prepared 100 mM of MgCl in PBS 1X then sterilized it by autoclave. But my buffer was precipitated. How to prevent this matter? Thanks

07 August 2018 3,736 0 View

How to calculate the amount of DNA strands for each goldnanoparticle?

I read one paper (Hai-Bo Wang, 2013/DOI: 10.1039/c3nj00328k), he claim that there are approximately 50 strands of DNA probe for each AuNP. But, when I check a table from this paper (please check...

06 July 2018 1,222 1 View

How much melting temperature and delta G are used by benchmarks to avoid DNA strand displacement?

I want to check the DNA strand displacement possibility of my DNA.

05 June 2018 531 0 View

Why the results of my marker (DNA Ladder 50 bp) bands turn to worse?

I use that DNA marker in same concentration of gel (10%) and running at 80 V for 80 minutes (everything is the same).

04 May 2018 7,935 8 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

Can chitosan alone can act as a reducing agent for Cu(0) nanoparticle sythesis?

[Synthesizing Cu nanoparticles without using chemical reductants]

01 March 2021 8,359 1 View

What are the new 2D nanomaterials were used for Silver nanoparticles stabilization?

In the stabilization of AgNPs are vital in current research interest. However, some of the 2D nano-materials like graphene oxide, MoS2, etc... previously used to stabilize it. But still they shown...

01 March 2021 2,207 3 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

How to check the uv-vis absorption of nanoparticle by surface plasmon resonance?

I try to check the uv-vis absorption of Indium nanoparticle. Now I am measuring in solution, can I measure it in the form of a film?

01 March 2021 930 3 View

Distribution of particles in metal matrix?

How would define the "good" and "poor" distribution of particles within the matrix. Is there a quantitative solution to assess the distribution of the particles, which then having denotation of...

28 February 2021 7,397 8 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View