In that case, as well as the effect of added 8 % sucrose solution.
I'm gratefull if somebody help me. Thank you
Nanoparticles for DNA delivery are often complex colloidal systems, and there are different routes to accomplish the combination or encapsulation of for example plasmids with liposomes. The buffer pH will have an effect on the overall charge of the nanoparticle, and thus can lead to instability near the isoelectric point unless other stability enhancing effects contribute significantly (i.e. steric stabilization, for example with PEG modification, sometimes termed "stealth" liposomes) to overcome the lack of charge repulsion.
Adding sucrose to the buffer may have an effect of improving stability, due to increased viscosity and the potential contribution of excluded volume (=steric) effects, however the significance of these will be sample dependent and may be influenced by the nanoparticle size.
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