I would check my DNA (length: 80 bp and 23 bp) using PAGE (polyacrylamide gel electrophoresis). The conditions are following these:

Fig 1. is 10 % gel, 100 V, 70 minutes

Fig 2. is 10 % gel, 100 V, 1 hour

Fig 3. is 10 % gel, 100 V, 2 hours

Fig 4. is 20 % gel, 100 V, 2 hours

Fig 5. is 20 % gel, 100 V, 3 hours

But, I have problem in my DNA Ladder when I check the results by GelDoc.

Can anyone help me??