Whether acetic acid (acetate glacial) can be replaced with hydrochloride (HCl) to reduce pH?

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Laurence Stuart Dawkins-Hall

No !!
You might obtain the right pH by using concentrated HCL but in effect you have added sodium chloride to a nascent solution of Tris acetate by using hydrochloric acid
You will need to make again

Katie A Burnette

You need to raise the pH from 7.4 to 8.2, not lower the pH. You'll have to add a base. Make sure your pH probe is properly calibrated, it seems very odd that your pH is lower than needed with a protocol that says to use glacial acid to adjust the pH.

Michael Toner

How did you adjust the pH up using HCl?

Follow procedure.