How to make 25 mM Tris acetate buffer containing 100 mM NaCl?

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Why did my lead nitrate solution precipitate?

Previously, I made Pb(NO3) stock in miliQ water and diluted into buffer. But I tried different way yesterday. I prepared 100 uM of Pb(NO3) in buffer 10 mM TA containing 50 mM NaCl, pH: 7.0-7.4 The...

01 February 2020 1,521 2 View

How much degree Celcius for boiling HAuCl4 solution?

Yesterday I synthesized 13 nm colloidal gold nanoparticles using hotplate stirrer (T= 300 C, s= 600 rpm), the solution boiled for a long time (until bubles out). Even it had already ~ more than 1...

03 April 2019 524 6 View

Actually how many the limitations of TAE or TBE to use (re-use) in gel electrophoresis?

Normally, I have fresh prepared TAE/TBE buffer. But sometimes I see one of my colleagues who re-use it max. 3 times, Is it corret or not? Does it have any effect to the result? Thanks

02 March 2019 674 3 View

How to make disulfide from thiol?

I have DNA (disulfide modifier) has already become the Thiol due to adding TCEP. So I want to make it be disulfide again. Thanks

07 August 2018 318 0 View

Whether acetic acid (acetate glacial) can be replaced with hydrochloride (HCl) to reduce pH?

I have 25 mM of Tris-acetate pH = 7.4 But, I need 25 mM of Tris-acetate pH = 8.2 for washing my DNA. I read a protocol, the adjuster should be using Acetate glacial. But, I have already adjusted...

07 August 2018 7,940 3 View

What is good idea to block AuNPs surface?

I need to block AuNPs surface (space between Thiol DNA - AunNPs) to prevent binding interaction with streptavidin.

07 August 2018 6,595 2 View

How to solve precipitated PBS; 100 mM MgCl ?

Yesterday I prepared 100 mM of MgCl in PBS 1X then sterilized it by autoclave. But my buffer was precipitated. How to prevent this matter? Thanks

07 August 2018 3,736 0 View

How to calculate the amount of DNA strands for each goldnanoparticle?

I read one paper (Hai-Bo Wang, 2013/DOI: 10.1039/c3nj00328k), he claim that there are approximately 50 strands of DNA probe for each AuNP. But, when I check a table from this paper (please check...

06 July 2018 1,222 1 View

How much melting temperature and delta G are used by benchmarks to avoid DNA strand displacement?

I want to check the DNA strand displacement possibility of my DNA.

05 June 2018 531 0 View

Why the results of my marker (DNA Ladder 50 bp) bands turn to worse?

I use that DNA marker in same concentration of gel (10%) and running at 80 V for 80 minutes (everything is the same).

04 May 2018 7,935 8 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Timeout error!! Processor in the loop (PIL) using STM32F4 Matlab 2018a?

Dear Researchers I am trying to perform a PIL simulation using STM32F4 Discovery board and comunication serial USB TO TTL. During simulation I receive the following timeout error: An error...

01 March 2021 2,327 1 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

Which database would give me this list of chemical components?

Dear colleagues: I would like to know if anyone knows of a database that gives me the parameters pH, K, K, Mg, P, C, N, Zn, Cu, Ni and Cd of common foods. I find a lot of information of proteins,...

26 February 2021 5,525 3 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

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I am trying to find how to know the density needed for a pva electrospinning in a 0.6mm neddle, the electrospinning will be from top to bottom and the problem I really have is the solution because...

25 February 2021 2,615 3 View