Which one the most correct, I read two protocols:
1. Dissolve Tris-base and NaCl in acetate acid
or
2. Dissolve Tris-base and NaCl in DI water, but adjust the pH using acetate acid (Not HCl).
Either may be OK depending on the application, but you need to specify the pH
Dear colleague,
I suggest procedure 2.
Actually, I use pH = 8.0 - 8.2
Thank you.
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