I dissolved my bacteriophage DNA in 1x TE buffer and stored at -20 °C. after 6 month i did not get DNA from that. at the same time i stored bacteriophage DNA with 10x TE buffer and i get DNA band from the stored sample.
Remember that most DNA modifying enzymes are Mg2+ or Ca2+ dependent, bivalent ions which are chelated by the EDTA. Trying to do something useful with your DNA afterwards could prove a big headache! Just store DNA in sterile TE or 10 mM Tris pH 7.5.
Remember that most DNA modifying enzymes are Mg2+ or Ca2+ dependent, bivalent ions which are chelated by the EDTA. Trying to do something useful with your DNA afterwards could prove a big headache! Just store DNA in sterile TE or 10 mM Tris pH 7.5.
TE buffer is much more common for DNA storage. TAE and TBE are widely used for electrophoresis, not for storage. But anyway DNA can't simpy diappear from your buffer. It can loose integrity or somthing else if you contaminate buffer some nucleases or in case of other factors but not dissapear. Result will depends on what technique you used for DNA precipitation from buffer. Note that in 10X buffer ion concentration is too high and can affect to DNA solubilizing. Any 10X buffer is a stock solution for storage of buffer, but not allowed using directly without necessary dilution rate. Check your buffer with DNA by spectrophotometer (nanodrop or other) in comparison to same concentration pure buffer without anything. In such way you will be able to chek DNA concentration in your solution
Concentration (µg/ml) = (A260 reading – A320 reading) × dilution factor × 50µg/ml
and also check purity of your DNA
DNA purity (A260/A280) = (A260 reading – A320 reading) ÷ (A280 reading – A320 reading)
By this method you can determine presence of any proteins as conaminants.
I get best results with long-term storing of DNA in sterile water or pellets in ETOH.
Its probably best to store your DNA in Qiagens DNA extraction elution buffer if you have some lying around your lab. Sterile water would do for short term storage but if you choose to store it for long periods, you can use LC/MS grade water.
You can use 10x TE for storage of DNA for long time it will not affect the DNA. But even TE buffer is more than enough to protect DNA from possible source of nucleases contamination. It also depends on the concentration of mg2+ in solution. If you dissolve DNA in 10x TE and use it for downstream reactions such as pcr or restirction digestion where mg2+ plays crucial role will be inhibited unless and untill it is diluted.
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