Ian N Oyaro

4 Questions 10 Answers 0 Followers

Questions related from Ian N Oyaro

When doing DNA extraction, I elute in 200 microlitres elution buffer. Do I then need to dry that sample to a smaller volume for optimal PCR?

Some colleagues tell to me to dry the sample to something like 20 microlitres so as to increase chances of picking out a bacterial DNA during PCR since I am using clinical samples to extract...

10 October 2014 6,242 15 View

When extracting bacteria DNA from clinical samples, over 90% of DNA extracted will be human. How can I reduce this contamination for downstream PCR?

Or does this high quantity of human DNA not affect PCR applications targetting the bacteria DNA where I am using 16s rRNA primers.

10 October 2014 8,130 5 View

Can someone share the protocol for Bacterial DNA extraction from a patients' sample, more preferably plasma or csf?

I am trying to optimize the extraction for downstream applications- amplification (conventional pcr) and sequencing

09 September 2014 7,993 2 View

For optimal Bacterial DNA extraction, is it really necessary to do an overnight proteinase K digestion coupled with lysozyme enzyme?

My research entails extracting Bacterial DNA from patients' samples (plasma and csf) as I explore to use molecular techniques to identify bacteria indicated in non-traumatic coma. As most bacteria...

09 September 2014 3,966 12 View