How to store a nitrocellulose membrane after western blot?

01 February 2020 9,382 3 View

How to reduce non-specific interaction without impairing antibody-epitope binding in immunohistochemistry (IHC) ?

Dear expertise, i stained frozen section lung tissue sample against virus protein, which should be detect virus infection. i trying to reduce non-specific interaction using serum (5% BSA in PBS)...

07 August 2019 9,401 2 View

What are the criteria need to apply post-doctoral position ?

Dear Expert, I would like to apply postdoctoral studies. 1. I am wondering how to apply for postdoctoral position especially in the US and Europe school. What are the procedures, I need to go...

06 July 2018 1,022 5 View

What are the OIE- Listed bacterial diseases mostly infecting the shrimp worldwide?

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03 April 2017 6,945 1 View

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I have done aquatic animal model experiments, which are treated with single, combination of different bacteriophage under laboratory condition, and I controlled bacterial infection by phages. I...

08 September 2016 8,024 11 View

Does anyone know where the facility of Pulsed-field gel electrophoresis for DNA sample in India?

05 June 2016 10,052 1 View

How do I concentrate the PCR products ?

10 November 2015 3,632 25 View

What is the basic method for bacteriophage SDS-PAGE and How do i get all phage structural protein bands ?

1. I am getting streaking with two big bands, two normal bands two/ three very small bands only between 70-40 kDa in 12 % SDS-PAGE gel (CBB strain). but i unable to remove streaking. Is there any...

08 September 2015 4,827 2 View

How to screen out for GAL gene containing bacteriophage from the plagues ?

03 April 2015 556 4 View

What is the basic method for RFLP and how do I get good digestion ?

Why I did not get good digestion for phage DNA? I need to determine the diversity of phage isolates, and the approximate genome size from the fragments generated from RFLP. My concentration of...

02 March 2015 7,534 11 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

Is there a method of determining feed digestibility without the need to re-pellet the feed?

I am aiming to determine the ADC values of a commercial fish feed, but I do not have a steam pelleter or extruder available to me. Ideally, the pellets could be coated with some inert marker....

28 February 2021 7,967 3 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View