How to store a nitrocellulose membrane after western blot?

01 February 2020 9,382 3 View

How to reduce non-specific interaction without impairing antibody-epitope binding in immunohistochemistry (IHC) ?

Dear expertise, i stained frozen section lung tissue sample against virus protein, which should be detect virus infection. i trying to reduce non-specific interaction using serum (5% BSA in PBS)...

07 August 2019 9,401 2 View

What are the criteria need to apply post-doctoral position ?

Dear Expert, I would like to apply postdoctoral studies. 1. I am wondering how to apply for postdoctoral position especially in the US and Europe school. What are the procedures, I need to go...

06 July 2018 1,022 5 View

What are the OIE- Listed bacterial diseases mostly infecting the shrimp worldwide?

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03 April 2017 6,945 1 View

Could anybody share their protocol for Mycobacterium bacteriophage isolation from aquatic environment?

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03 April 2017 9,679 1 View

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I have done aquatic animal model experiments, which are treated with single, combination of different bacteriophage under laboratory condition, and I controlled bacterial infection by phages. I...

08 September 2016 8,024 11 View

How do I concentrate the PCR products ?

10 November 2015 3,632 25 View

What is the basic method for bacteriophage SDS-PAGE and How do i get all phage structural protein bands ?

1. I am getting streaking with two big bands, two normal bands two/ three very small bands only between 70-40 kDa in 12 % SDS-PAGE gel (CBB strain). but i unable to remove streaking. Is there any...

08 September 2015 4,827 2 View

How to screen out for GAL gene containing bacteriophage from the plagues ?

03 April 2015 556 4 View

What is the basic method for RFLP and how do I get good digestion ?

Why I did not get good digestion for phage DNA? I need to determine the diversity of phage isolates, and the approximate genome size from the fragments generated from RFLP. My concentration of...

02 March 2015 7,534 11 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

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02 March 2021 3,060 3 View

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02 March 2021 932 1 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View