1. I am getting streaking with two big bands, two normal bands two/ three very small bands only between 70-40 kDa in 12 % SDS-PAGE gel (CBB strain). but i unable to remove streaking. Is there any method to remove streaking?.
2. I need to isolate from both Myoviridae and Shipoviridae phage
3. Protein concentration was 43 ± 7µg/ml.
SDS-PAGE was invented by U.K. Laemmli for separating protens in T4 bacteriophage (Laemmli UK Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227 (5259): 680–685, http://www.nature.com/nature/journal/v227/n5259/abs/227680a0.html). So, I think this is the most basic technique.
Nattan,
I have not worked with your phages before, but I have had luck with a very fast cooling in -80 degrees C freezer before loading into the gel to decrease streaking.
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