Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?
I've been trying to do my calculations related to optimization of excited state of ion CH5+ using method TD-DFT with M06-2X and aug-cc--pVTZ. My input file is as shown...
03 January 2021 2,148 0 View
Hello! I am performing preliminary analyses on my data set (N= 187 heterosexual couples). When examining the summary of missing values, SPSS indicates that only 0.16% of my data is missing....
01 December 2020 5,562 13 View
I am working on research where I have 66 participants who perform an exercise every day, and then they need to respond to 14 questions with a Likert scale (1-10). They performed this exercise for...
20 November 2020 4,682 6 View
I am trying to start a protocol involving bilateral cannula implantation in specific brain regions in mice (mPFC, hippocampus). For that, I need to buy an anesthesia system. I found many...
06 November 2020 3,613 3 View
Dear all, I am measuring changes in fluorescence lifetime of my chromophore in cells expressing also GFP and I would need to filter off the GFP signal. After playing with the parameters in...
11 August 2019 5,297 3 View
I am trying to find the correlation/regression between these two variables: Social media usage (measured by 10 items) (total scale score 50) Materialism (measured by 9 items) (total scale score...
10 April 2019 4,123 4 View
Hi! Can you recommend any company (or at least a research-team) where I can order a microplate covered with peptides according to my needs? I need it for immunological assays. Thanks for all...
12 February 2019 5,959 2 View
I am researching whether dance can be considered a language, and different languages effects on movement quality. i am interested in how dancing to spoken word, interpreting it affects dancers...
14 August 2018 4,051 11 View
What is the best way to isolate genomic DNA from water? Using the ready kit centrifuge a sample of water to obtain the sediment of microorganisms or maybe filter water and isolate DNA from the...
07 August 2018 2,397 8 View
I am facing a problem I can not find where to purchase MAD109 (M109) cells. Would someone be so kind and help me or even share it with me?
28 March 2018 9,101 2 View
Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...
03 March 2021 1,499 2 View
Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...
03 March 2021 6,299 3 View
Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...
01 March 2021 2,215 2 View
Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.
01 March 2021 9,952 3 View
I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...
01 March 2021 9,015 2 View
I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...
26 February 2021 7,129 3 View
Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...
24 February 2021 5,749 3 View
Hi everyone! I try to detect VDR protein (~48 kDa) using Western Blot. After SDS-PAGE using 10% MP TGX Stain-Free Gel from BioRad I transferred the protein onto an metOH activated PVDF membrane...
23 February 2021 6,266 3 View
Hello everyone I would like to improve the buffer capacity of seawater (pH ~8) in order to dissolve as much CO2 as possible without dropping the pH below 7.5 (a range between 7 - 7.5 is...
17 February 2021 2,797 2 View
I use 1% BSA in 10 mM phosphate buffer (pH 7.4) solution when striping my nitrocellulose membranes with antigens and antibodies. However, I always observe deformed flow when my sample or running...
16 February 2021 4,224 3 View