Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?
How to change the displayed full article text to its corrected version? In the file on the page of the journal where I published the article, there was an error in the text, the table is...
30 July 2024 3,229 2 View
Hi, I came across an issue when graphical results do not match the optimal reconstruction in the list section in RASP v4.0. In the graphical section, I would have 50:50 division of a nod pie,...
02 December 2023 5,082 1 View
What software or app would you recommend for a beginner, who would like to learn the basics of designing a wireframe?
22 September 2023 2,941 4 View
My question concerns acceptability ratings in linguistics. Following the advice in i.a. Cowart (1997) and Bross (2019), I included filler sentences in my questionnaires. Some fillers are also...
07 July 2023 2,718 0 View
Hi, I'm trying to display distribution of haplotypes of a population. I have already created haplotype networks with the pie charts coloured by site (i.e. a basic haplotype network). I wanted to...
09 May 2023 4,408 1 View
We are currently doing research for our subject Ethnic minorities in Europe and we are looking for some respondents. Our seminar paper is focussed on Catalans in France.
10 March 2023 9,432 0 View
Hello, I've done a CFA and want to compare a first-order model with a second-order model. Can anyone give me a hint on how to do this in AMOS? I found many tutorials, but the comparisons presented...
06 March 2023 903 2 View
Hi, I have an issue with AMOS, so after preparing the diagram and analyzed the estimates, in the Estimates -> scalars -> there are missing the correlations and variances, in a first analysis...
05 January 2023 7,608 1 View
Hello, I am designing the experiment on application of Boron (H3BO3) on seedlings and checking if there's any result in blind plants. Most teams use units kg/ha, only few mg/L. I would like to...
06 November 2022 8,875 2 View
Hi everyone, I would like to ask how I can create a this kind of picture and where I can get a data of values? I have gaussian and gaussview programs. Thank you so much for your help.
14 October 2022 142 3 View
Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...
06 August 2024 5,370 2 View
Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...
05 August 2024 3,781 2 View
Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC
01 August 2024 965 3 View
I want to preserve the sample leaves for the LM study of the stomata and conidia analysis. Whether 2.5% glutaraldehyde in 0.05 M phosphate buffer with pH 7 can be applied for preservation.
29 July 2024 8,559 0 View
Hello everyone! How can i prepare EDTA buffer solution (pH 8.2) to determine GSH levels? Thank you in advance.
29 July 2024 4,372 1 View
// interested in the difference between floating events and short circuits.
22 July 2024 6,565 0 View
Our lab has extracted bacterial DNA samples using Qiagen DNeasy kit and eluted the samples in AE buffer. However, this buffer contains 0.5mM EDTA not suitable for downstream application. Any ideas...
13 July 2024 1,673 3 View
Commonly, we prepare 1.0% of agarose gel with 0.4g agarose powder and 40ml 1X TAE buffer. if I would like to prepare a 1.2%% of agarose gel, is it I need to add 0.48g agarose powder and 120ml 1X...
12 July 2024 2,058 5 View
I performed CV of Cu-MOF in a buffer solution of pH 8, and we did not find any peak corresponding to Cu2+ to Cu+. What is the reason behind this? Please give your valuable suggestions.
08 July 2024 2,386 4 View
Dear colleagues, We are trying to do FISH on mice PDEC metaphase spreads. We use homemade biotin labelled probes, synthetized using a nick translation kit. For hybridization, initially we were...
08 July 2024 8,685 3 View