Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

12 August 2024 1 5K Report

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

Arshed Kadim

Yes you can, but here the case in reverse sequences is considered a copy to produce nucleic acids and not as a reverse sequence.

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