Sachin A. More

7 Questions 106 Answers 0 Followers

Questions related from Sachin A. More

Can somebody tell me where will I get the use manual of SAS statistical analysis software?

I want to learn SAS a statistical analysis software for data analysis. I am new to this software and I am searching for step wise user guide to begin statistical data analysis. Please share with...

08 August 2016 9,669 0 View

Why a previously protein expressing same gene construct in pET vector (BL21 host) is not showing protein expression?

I am facing problem in protein expression from the gene cloned in pET28 vector (BL21 host) which was previously expressing happily. Now I am trying to induce protein from same construct in same...

06 June 2015 3,337 11 View

How do I identify correct recombination events worked out using RDP 4 software?

I am trying to find out recombination among viral sequences using RDP 4 software. With the aid of this software I identified two about 30 recombination events. Out these 30 recombination events...

03 March 2015 7,726 17 View

Can we get more than one dissociation curve during real time PCR for one sample contaminated with genomic DNA from other sources ?

I just want to know whether the real time PCR will show different denaturation curves for single sample contaminated with different genomic DNA from different organismal sources? Suppose I have...

04 April 2014 8,554 5 View

Why is the 16s rDNA sequence from endosymbiotic bacteria not getting amplified by PCR?

I am trying to amplify via PCR the 16s rDNA sequence from endosymbiotic bacteria of insects. I tried different concentrations and dilutions of the template. Initially, it was showing good...

02 February 2014 8,240 3 View

What is the optimum temperature for pollen germination, pollen viability, and stigma receptivity in cotton flowers?

10 October 2013 980 0 View

Is there any problem in ligation of sma1 and ecor1 insert with vector cut with same res. ezymes?

10 October 2013 3,228 3 View