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Questions related from Nattan Stalin
I would like to use my western blot membrane after re-probing (mild stripping) but I don’t know if will be able to use it often. Is there a way to store nitrocellulose membrane over the months...
02 February 2020 9,675 3 View
Dear expertise, i stained frozen section lung tissue sample against virus protein, which should be detect virus infection. i trying to reduce non-specific interaction using serum (5% BSA in PBS)...
08 August 2019 9,516 2 View
Dear Expert, I would like to apply postdoctoral studies. 1. I am wondering how to apply for postdoctoral position especially in the US and Europe school. What are the procedures, I need to go...
07 July 2018 1,143 5 View
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04 April 2017 7,051 1 View
04 April 2017 9,799 1 View
I have done aquatic animal model experiments, which are treated with single, combination of different bacteriophage under laboratory condition, and I controlled bacterial infection by phages. I...
09 September 2016 8,145 11 View
To know various bacteriophage DNA genotyping (DNA size)
06 June 2016 10,166 1 View
I have PCR product for sequencing and its too low concentration. How do I get more yield of PCR products.
11 November 2015 4,106 25 View
1. I am getting streaking with two big bands, two normal bands two/ three very small bands only between 70-40 kDa in 12 % SDS-PAGE gel (CBB strain). but i unable to remove streaking. Is there any...
09 September 2015 4,952 2 View
I have been more interested to read above-mentioned topic, give me your valuable answer with research articles. I am working with Vibrio phage. With Thanks and Regards, -Stalin.n
04 April 2015 679 4 View
Why I did not get good digestion for phage DNA? I need to determine the diversity of phage isolates, and the approximate genome size from the fragments generated from RFLP. My concentration of...
03 March 2015 7,650 11 View
Dear experts, I have done RAPD gradient PCR at different temperature by using takara pcr master mix without additional Taq DNA Polymerase and dNTPs. There is only one band upper ladder all the...
03 March 2015 6,771 10 View
I dissolved my bacteriophage DNA in 1x TE buffer and stored at -20 °C. after 6 month i did not get DNA from that. at the same time i stored bacteriophage DNA with 10x TE buffer and i get DNA band...
02 February 2015 6,921 8 View
Dear phage experts, I recently tried TEM analysis for my isolated phage (H. W. Ackermann, 2011 protocol), but I could not get images and results. Can anyone help me to analyze my phage by...
01 January 2015 6,203 16 View
Respected Phage experts, Give me your valuable experience/suggestion to detect the bacteriophage by Real Time PCR.
12 December 2014 1,695 0 View
I performed TEM analysis for my bacteriohage.repetitively I got only salts images how to avoid the presence of salts during TEM analysis.
12 December 2014 5,923 7 View
I have Isolated VIbrIo bacteriophage genomic DNA by phenol/chloroform method, I found a thick DNA band with more streak and herewith I have attached my page DNA gel picture, I need suggestion for...
07 July 2014 7,398 7 View
07 July 2014 5,784 2 View
I have more than 10 Vibrio species in my lab. When I started purification (streaking) on TCBS plates, they were a yellow color within 24 hour incubation at 35˚C, when I continued their incubation...
05 May 2014 9,227 7 View
see above
03 March 2014 2,393 7 View
Need suggestion, good ideas and research articles
06 June 2013 7,897 0 View
06 June 2013 8,041 4 View
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05 May 2013 5,929 5 View
Dear experts, I would like to work on THP-1 as adherent cells without changing its natural. Is there any method or protocol for develop adherence cell from suspension cell lines?
01 January 1970 3,488 2 View
Dear expertise, Could someone can share with me a good protocol for flow cytometry based assay for titrating virus.
01 January 1970 1,851 1 View
