Why didnt methanol boil at 90 degree celcius ?

i extracting the phytochemicals from the grounded root samples using methanol as solvent by soxhlet apparatus. i used oil bath to heat the methanol since my heating mantle wasn't available at the...

03 April 2019 2,451 2 View

Issue with PCR amplification of an amplicon ?

I have done a 16S rDNA amplification of a metagenomic sample. I got a clear band near 1.5kb in the gel. Later on purification and again when we do the same PCR with the exact same primers and...

01 February 2019 7,351 6 View

Is it necessary to dissolve the methanolic extract in DMSO before checking the antifungal activty of any plant extract?

so, basically i had to do an anti-fungal assay using a plant extract which was extracted using methanol. then i dissolved the extract in methanol to bring 200mg/ml working concentration. I made...

01 February 2019 5,582 5 View

Can somebody please suggest me with an article to assess the antagonistic activity of a fungi on another fungi ?

i am currently looking for some protocols to be followed to assess the antifungal activity of a new fungus against a pathogenic fungi. i have no idea what fungi it is. so, i dont know what type of...

01 February 2019 3,163 17 View

What does the superscripted alphabet in the data table represent in some research articles ?

recently i encountered some research papers where they have super-scripted some alphabets on top of the values. can somebody please explain me what they are trying to infer. i have attached the...

01 February 2019 2,277 0 View

Is it ok to use McFarland turbidity standards for MIC determination of a plant extract against fungi ?

I have prepared a fungal suspension by scrapping out the fully grown fungal culture from the agar plate and homogenised them. i refered some of the literatures where they have used McFarland...

01 February 2019 3,762 3 View

Can someone help me with GC-MS results analysis?

i have recently done a GCMS analysis for the methanolic extracts of plant samples. i am not sure how to interpret the results. can someone help me with any protocol or procedure on how to begin...

01 February 2019 9,491 8 View

How to grow both bacteria and a fungi together in a single media ?

Basically, i am trying to find the antagonistic activity of the bacteria and a pathogenic fungi. what could be the possible way to culture them if the incubation temp is 37 degree celsius for the...

09 October 2018 1,059 2 View

Does nitrocellulose membrane that is used for the southern blotting technique have more binding capacity than the Silica matrix of Dna purification ?

the same procedure used for the DNA elution and purification. i need to know whether the nitrocellulose membrane carries any affinity towards DNA during the blotting like the silica matrix does...

07 August 2018 804 4 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

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02 March 2021 3,060 3 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

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28 February 2021 9,936 2 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View